金属蛋白酶类及层粘连蛋白受体与人黑色素瘤细胞侵袭转移的关系

目的揭示金属蛋白酶类(MMPs)及层粘连蛋白受体(LN-R)与人黑色素瘤细胞侵袭转移的关系，并探讨MMPs及IN-R用以判断肿瘤细胞侵袭转移的可能性。方法通过流式细胞术(FCM)定量研究和蛋白酶活性分析(Zymography)，对具有不同潜在转移能力的人黑色素瘤细胞系(WM35，WM134b，WM983a，WM451)进行MMPs及瘤细胞表面67000LN-R的荧光阳性率和全部细胞的平均荧光强度测定。结果早期WM35不产生MMPs；WM1341b仅产生MMP2，不产生MMP9；进展期WM983a和远处转移瘤株WM451既产生MMP2又产生MMP9。瘤细胞表面67000LN-R的荧光阳性率和全部细胞的平均荧光强度大小顺序为WM451>WM983a>WM1341b>WM35。结论MMPS和LN-R与人黑色素瘤细胞侵袭转移能力的获得之间关系密切，并可作为较特异的肿瘤侵袭转移标记物应用于肿瘤研究与治疗中。

Relationship between metalloproteinases, Laminin receptor and invasivemetastasis of Human melanoma cells

Aim: To explore the relation between metalloproteinases(MMPs),Laminin receptor(LN-R) and invasive metasta- sis of human melanoma cell and probe their probability of judging tumor invasive metastasis. Methods: Through the quantitative analysis of FCM and Zymography, studied human melanoma cell lines with different metastatic potenials. Results: At early stage, WM35 didn't produce MMPs, and WM134lb produced only MMP-2, but no MMP-9. Both WM983a at progressive stage and WM451 produced MMP-2 and MMP-9.The fluorescent positive the of 6700 0 LN-R on the surface of melanoma cells and the average fluorescent intensity were WM451 > WM983a > WM1341b > WM35. Conclusion: MMPs and LN-R closely correlate with inva- sive metastasis ability of human melanoma cell lines and may be as a specific mark of tumor invasive metastasis when used in study and treatment of tumor.