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| IL-4/IL-10诱导的树突状细胞对类风湿性关节炎的作用 | |
| 引用本文： | 聂瑛洁,周晓泉,袁军,安宇,肖林生. IL-4/IL-10诱导的树突状细胞对类风湿性关节炎的作用[J]. 中国免疫学杂志, 2011, 27(12): 1104-1108,1112. DOI: 10.3969/j.issn.1000-484X.2011.12.12 |
| 作者姓名： | 聂瑛洁 周晓泉 袁军 安宇 肖林生 |
| 作者单位： | 1. 贵州省人民医院中心实验室,贵阳,550000 2. 贵州省血液中心,贵阳,550000 3. 贵州省贵阳医学院,贵阳,550001 |
| 基金项目： | 贵州省卫生厅基金(gzwkj2010-1-009); 贵州省科技厅基金黔科合SY[2010]3131资助 |
| 摘 要： | 目的:探讨经IL-4/IL-10诱导的树突状细胞(DC)对类风湿性关节炎的作用。方法:用Percoll分层离心法从脾脏细胞分离得到DC后,用IL-4或IL-10或IL-4+IL-10进行诱导。用未经诱导和诱导过的DC对类风湿性关节炎大鼠模型进行干扰。SD鼠设为CIA模型组,DC对照组与DC试验组。用ELISA法检测细胞因子与抗体水平,用MTT法检测细胞增殖情况,对鼠爪关节行病理学检测。结果:DC对照组的临床症状评分,病理改变评分,细胞增殖能力,血清中抗胶原抗体及细胞因子水平与CIA模型组的差异无统计学意义。试验组中,注射IL-10-DC能改善CIA鼠的滑膜炎症情况;在初次免疫后第5天注射IL-4-DC能减轻CIA鼠的滑膜炎程度;注射IL-4+IL-10-DC无明显的保护作用。结论:适时注射IL-4或IL-10诱导的DC,对实验性类风湿性关节炎具有保护作用。 |
| 关 键 词： | 树突状细胞 自身免疫性疾病 类风湿性关节炎 白细胞介素 |
The potential of IL-4/IL-10 pre-treated dendritic cells on rheumatoid arthritis |
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| NIE Ying-Jie,ZHOU Xiao-Quan,YUAN Jun,AN Yu,XIAO Lin-Sheng. The potential of IL-4/IL-10 pre-treated dendritic cells on rheumatoid arthritis[J]. Chinese Journal of Immunology, 2011, 27(12): 1104-1108,1112. DOI: 10.3969/j.issn.1000-484X.2011.12.12 | |
| Authors: | NIE Ying-Jie ZHOU Xiao-Quan YUAN Jun AN Yu XIAO Lin-Sheng |
| Affiliation: | NIE Ying-Jie,ZHOU Xiao-Quan,YUAN Jun,AN Yu,XIAO Lin-Sheng.The Clinical Laboratory Centre,Guizhou Provincial People's Hospital,Guiyang 550000,China |
| Abstract: | Objective:To investigate the potential therapeutic function of IL-4/IL-10 pre-treated dendritic cells(DC) in treating rheumatoid arthritis.Methods:DCs were isolated from total spleen cells by centrifugation at 3 000 r/min for 30 min in Percell.Then they were treated with IL-4/IL-10/IL-4+IL-10.SD rats were divided into three groups,DC control,Collagen induced arthritis(CIA) model and DC experimental group.DCs pre-treated with different cytokines were injected intravenously to treat SD rats.The level of cytok... |
| Keywords: | Dendritic cells(DC) Autoimmune diseases Rheumatoid arthritis(RA) Interleukin(IL) |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |