| 抗人CD28激发性单抗对T细胞淋巴瘤的抑制作用研究 |
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| 引用本文： | 胡玲玲,陈昌友,张益红,郭静雅,陆海波,旬神美,邱玉华. 抗人CD28激发性单抗对T细胞淋巴瘤的抑制作用研究[J]. 中国免疫学杂志, 2011, 27(10): 880-883. DOI: 10.3969/j.issn.1000-484X.2011.10.004 |
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| 作者姓名： | 胡玲玲 陈昌友 张益红 郭静雅 陆海波 旬神美 邱玉华 |
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| 作者单位： | 1. 南京医科大学第二附属医院检验科,南京,210011
2. 苏州大学医学部基础医学与生物科学学院,苏州,215123 |
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| 基金项目： | 南京医科大学第二附属医院青年科技启动基金 |
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| 摘 要： | 目的:研究鼠抗人CD28单克隆抗体(克隆号:2F5)对T淋巴细胞瘤的抗肿瘤效应,为抗体介导肿瘤靶向治疗提供新的方法和思路。方法:流式细胞术检测鼠抗人CD28单克隆抗体2F5对人T淋巴瘤细胞株Jurkat细胞表面膜型CD28分子的识别;MTT法检测2F5对Jurkat细胞体外增殖的影响;将Jurkat细胞株接种于裸鼠腋下,建立Jurkat细胞荷瘤裸鼠模型;经瘤内多点注射2F5(注射剂量1μg/mm3),逐日观察肿瘤的大小和裸鼠的生存状态。结果:2F5能够识别Jurkat细胞表面CD28分子,阳性结合率可达93.37%;2F5与Jurkat细胞共培养后,显微镜下观察可见胞质中出现黑色颗粒,胞膜破裂,细胞的折光性和立体感减弱;MTT法分析结果显示,抗CD28单抗对Jurkat细胞的体外增殖具有明显的抑制作用;将Jurkat细胞接种35只裸鼠(成瘤率达70%);经瘤内多点注射2F5,35天后荷瘤小鼠体内肿瘤的生长速率明显降低;而生理盐水对照组肿瘤生长无明显改变。结论:鼠抗人CD28单克隆抗体2F5通过与Jurkat细胞表面膜型CD28分子结合对其增殖具有明显的抑制作用,提示CD28分子可作为治疗T淋巴瘤的一个潜在靶点,抗体对治疗CD28分子阳性的肿瘤具有潜在临床应用价值。
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| 关 键 词： | CD28 鼠抗人CD28单克隆抗体 T淋巴细胞瘤 |
Provocative mouse-anti-human monoclonal antibody against human CD28 inhibits growth of T cell lymphoma |
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| HU Ling-Ling,CHEN Chang-You,ZHANG Yi-Hong,GUO Jing-Ya,LU Hai-Bo,XUN Shen-Mei,QIU Yu-Hua. Provocative mouse-anti-human monoclonal antibody against human CD28 inhibits growth of T cell lymphoma[J]. Chinese Journal of Immunology, 2011, 27(10): 880-883. DOI: 10.3969/j.issn.1000-484X.2011.10.004 |
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| Authors: | HU Ling-Ling CHEN Chang-You ZHANG Yi-Hong GUO Jing-Ya LU Hai-Bo XUN Shen-Mei QIU Yu-Hua |
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| Affiliation: | HU Ling-Ling,CHEN Chang-You,ZHANG Yi-Hong,GUO Jing-Ya,LU Hai-Bo,XUN Shen-Mei,QIU Yu-Hua.The Second Affiliated Hospital of Nanjing Medical University,Laboratory Department,Nanjing 210011,China |
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| Abstract: | Objective:Effect of mouse-anti-human Mab against human CD28,clone No.2F5,(hereafter referred as 2F5) on inhibition of T cell lymphoma was investigated,which may possibly provide creative methods in antibody mediated targeted therapy.Methods:Recognition of 2F5 with surface molecule CD28 on Jurkat cell,one of T lymphoma cell lines,was examined by flow cytometry.Effect of 2F5 on Jurkat proliferation in vitro was examined through MTT assay.Jurkat cells were inoculated at armpit of naked mouse in order to establ... |
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| Keywords: | CD28 Mouse-anti-human monoclonal antibody CD28 T cell lymphoma |
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