何首乌和夜交藤药材指纹图谱研究与评价

目的 建立何首乌、夜交藤药材指纹图谱分析方法,并依据指纹图谱对两种药材进行比较,为科学评价与有效控制何首乌、夜交藤药材质量提供新方法.方法 何首乌和夜交藤分别用甲醇和醋酸乙酯超声处理,提取液用HPLC进行测定.HPLC法采用C18色谱柱,乙腈-0.1%磷酸梯度洗脱,检测波长分别为265和290 nm,体积流量1mL/min,柱温30℃.结果 建立了何首乌、夜交藤药材HPLC指纹图谱,并对不同产地何首乌、夜交藤药材进行了相似度比较.测定结果显示不同产地药材存在较大差异,化学成分组成及质量分数存在一定差异.结论 所建立的方法简便、可靠,可用于不同产地何首乌、夜交藤药材的指纹图谱测定和质量评价,为全面有效控制何首乌药材的内在质量提供了依据.

Fingerprints for Radix Polygoni Multiflori and Caulis et Folium Polygoni Multiflori

Objective To establish HPLC fingerprints for Radix Polygoni Multiflori and Caulis et Folium Polygoni Multiflori,to compare the fingerprints of the above two crude drugs collected from different habitats,and to provide a new method for scientific evaluation and quality control of them.Methods Radix Polygoni Multiflori and Caulis et Folium Polygoni Multiflori were extracted by supersonic wave with methanol and ethyl acetate.The chromatographic procedure for fingerprints was carried out using C18 as an analytic column,gradient eluted with a mixture consisting of acetonitrile and 0.1% H3PO4.Results This experiment established a specific method for the quality control of Radix Polygoni Multiflori and Caulis et Folium Polygoni Multiflori,and compared the similarity of Radix Polygoni Multiflori with Caulis Polygoni Multiflori from different habitats.Conclusion This method is very simple,reliable,and can be used for the fingerprint assay and quality control of Radix Polygoni Multiflori and Caulis et Folium Polygoni Multiflori.It provides a new method as a comprehensive quality control item for Radix Polygoni Multiflori and Caulis et Folium Polygoni Multiflori.