A forensic case study for body fluid identification using DNA methylation analysis |
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| Affiliation: | 1. Forensic DNA Division, National Forensic Service, 10, Ipchun-ro, Wonju si., Gangwon-do 26460, Republic of Korea;2. Department of Forensic Sciences, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu Suwon-si, Gyeongi-do 16419, Republic of Korea;1. Forensic Science Laboratory, Ehime Prefectural Police Headquarters, 2-2 Minamihoribatacho, Matsuyama, Ehime 790-8573, Japan;2. Department of Legal Medicine, Ehime University Graduate School of Medicine, Shitsukawa, Toon, Ehime 791-0295, Japan;1. Department of Forensic Medicine, Kyoto University Graduate School of Medicine, Kyoto, Japan;2. Forensic Science Laboratory, Kyoto Prefectural Police Headquarters, Kyoto, Japan;3. Department of Urology, Kyoto University Graduate School of Medicine, Kyoto, Japan;4. Ichioka Urological Clinic, Symphonia‐Oike1F, Higashinotoin‐Nijo Sagaru, Kyoto, Japan;1. Department of Chemistry and Biochemistry, Florida International University, Miami, FL 33199, USA;2. Faculty of Biosciences, Laboratory of Human and Molecular Genetics, PUCRS, 90619-900 Porto Alegre, Brazil;3. School of Criminal Justice, University of Southern Mississippi, Hattiesburg, MS 39406, USA;4. DNA Laboratory, Broward County Sheriff''s Office, Fort Lauderdale, FL 33301, USA;1. Institute of Forensic Medicine, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu, China;2. Institute of Basic Medicine, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu, China |
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| Abstract: | Recently, a method of identifying body fluids using DNA methylation has been developed (Frumkin et al., 2011). An existing multiplex assay using 9 CpG markers could differentiate 5 body fluids: semen, blood, saliva, menstrual blood, and vaginal fluid. To validate this technique, we evaluated the previously described body fluid identification method by means of single base extension (SBE). DNA methylation was applied to 22 samples in 18 forensic cases; seven of these were semen, three were blood, eight were saliva, three were vaginal fluid, and one was menstrual blood. Total of 18 samples were tested, the DNA methylation profiles were coincident from preliminary tests (acid phosphatase (AP), leucomalachite green (LMG, Sigma Aldrich, St Louis, MO, USA) and SALIgAE®) except one sample which displayed an all-negative result. After applying the DNA methylation method to forensic samples, we determined that it could be very useful for differentiating vaginal secretions from menstrual blood, for which there is no conventional preliminary testing method. |
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| Keywords: | Forensic genetics DNA methylation Bodyfluid identification |
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