| 重组人钙调素对人脐静脉内皮细胞增殖作用的影响 |
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| 引用本文： | 朱红,李晓军,武建国.重组人钙调素对人脐静脉内皮细胞增殖作用的影响[J].现代免疫学,2002,22(4):275-277. |
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| 作者姓名： | 朱红 李晓军 武建国 |
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| 作者单位： | 1. 徐州第四人民医院核医学科,徐州 221009
2. 南京军区南京总医院解放军医学检验中心,南京,210002 |
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| 基金项目： | 江苏省自然科学基金(BK95141307) |
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| 摘 要： | 本文探讨外源性重组人钙调素(recombinant human calmodulin,rhCaM)对人脐静脉内皮细胞(HUVEC)增殖作用的影响。采用基因重组技术在大肠杆菌DH5α中高效表达并纯化rhCaM。将HUVEC接种于96孔培养板,加入不同浓度rhCaM,用MTT比色法检测rhCaM对HUEVC细胞增殖作用的影响。结果表明rhCaM在0.04～0.4μg/ml浓度范围内可促进HUEVC的增殖,促增殖作用随细胞培养密度的增加而减弱,也与培养基中新生小牛血清的含量密切相关。故rhCaM对HUEVC增殖具有促进作用。
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| 关 键 词： | 钙调素 基因重组 细胞增殖 人脐静脉内皮细胞 |
| 文章编号： | 1001-2478(2002)04-0275-03 |
| 修稿时间： | 2001年6月1日 |
Effect of Recombinant Human Calmodulin on Proliferation of Normal Human Umbilical Vein Endothelial Cells |
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| ZHU Hong,LI Xiao-jun,WU Jian-guo.Effect of Recombinant Human Calmodulin on Proliferation of Normal Human Umbilical Vein Endothelial Cells[J].Current Immunology,2002,22(4):275-277. |
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| Authors: | ZHU Hong LI Xiao-jun WU Jian-guo |
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| Abstract: | To investigate the influences of recombinant human calmodulin (rhCaM )on the proliferation of human umbili-cal vein endothelial cells (HUVEC ) . The rhCaM protein was expressed efficiently by using the genetic recombination techniques. The HUVEC cells were seeded into 96 well plates and cultured with rhCaM, and the celi proliferation rate determined by MTT assay. It was found that the proliferation rates of HUVEC were increased by use of rhCaM at a con-centration range of 0.04 -0.4 μg/ml with a reducing effect by increasing cell density. It concludes that the extracellular rhCaM may promote the proliferation of the HUVEC cells. |
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| Keywords: | extracellular calmodulin gene recombination celi proliferation HUVEC |
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