| 大鼠海马神经元大电导钙激活钾通道的全细胞电流记录 |
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| 引用本文： | 张宇,高秀萍,李建国,郑肇青,张策.大鼠海马神经元大电导钙激活钾通道的全细胞电流记录[J].山西医科大学学报,2006,37(4):341-343. |
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| 作者姓名： | 张宇 高秀萍 李建国 郑肇青 张策 |
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| 作者单位： | 山西医科大学神经生物学研究室,太原,030001 |
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| 摘 要： | 目的 探讨记录全细胞的大电导钙激活钾通道的技术方法。方法 首先酶解急性分离大鼠海马神经元；利用全细胞膜片钳技术记录大电导钙激活钾电流。结果 可记录到一系列快速的外向钾离子电流。结论 所记录到的外向钾离子电流中，快速出现并很快减小的呈尖峰样的瞬变外向电流主要由大电导的钙激活钾电流组成。
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| 关 键 词： | 大电导钙激活钾通道 细胞分离 全细胞膜片钳 神经元 |
| 文章编号： | 1007-6611（2006）04-0341-03 |
| 收稿时间： | 2006-03-08 |
| 修稿时间： | 2006-03-08 |
Large-conductance Ca2 + -activated K+ currents in rat hippocampal CA1 neurons recorded by whole cell clamp technique |
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| ZHANG Yu, GAO Xiu-ping, LI Jian-guo, et al.Large-conductance Ca2 + -activated K+ currents in rat hippocampal CA1 neurons recorded by whole cell clamp technique[J].Journal of Shanxi Medical University,2006,37(4):341-343. |
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| Authors: | ZHANG Yu GAO Xiu-ping LI Jian-guo |
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| Institution: | Dept of Neurobiology, Shanxi Medical University, Taiyuan 030001, China |
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| Abstract: | Objective To explore a method for the whole currents record of a large-conductance Ca~(2 )-activated K~ currents in rat hippocampal CA1 neurons.Methods After rats hippocampal CA1 neurons were isolated by protease,the large-conductance Ca~(2 )activated K~ currrents were recorded in rats hippocampal neurons by whole cell clamping technique.Results A very strong outward currents appeared.Conclusion The outward currents are composed of transient outward currents with multiple compounds were recorded and sustained outward currents.And Ca~(2 )-activated K~ channels mediate a major part of the transient outward. |
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| Keywords: | large-conductance Ca~(2 )-activated K~ currents cell separation whole cell clamp technique neurons |
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