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| C57BL/6J小鼠主要器官的组织学数据库的建立 | |
| 引用本文： | 邓玉江,陈俊颖,邱全光,王勇,魏泓. C57BL/6J小鼠主要器官的组织学数据库的建立[J]. 局解手术学杂志, 2010, 19(3): 166-167 |
| 作者姓名： | 邓玉江 陈俊颖 邱全光 王勇 魏泓 |
| 作者单位： | 西南大学生命科学院;解放军第324医院病理科;第三军医大学信息网络管理中心;第三军医大学基础医学部实验动物学教研室; |
| 基金项目： | 国家科技部“十一五”科技支撑计划课题(2006BAI23B04);;国家“973”课题(2007CB513007) |
| 摘 要： | 目的建立C57BL/6J小鼠主要器官的组织学数据库。方法选取C57BL/6J小鼠雌性10只,雄性10只,将其麻醉致死,解剖切取心、肝、脾、肺、肾、胃等45个器官和组织,用10%的中性缓冲福尔马林固定,石蜡切片,常规HE染色,用显微镜观察,图像采集,用计算机对图像进行标注。采用SQLSEVER2000数据库管理系统,C#编程语言,B/S（浏览器/服务器）为系统构架。结果解剖20只C57BL/6J小鼠主要器官和组织,制做了1 589张切片,观察采集了13 578张图片,对1 563张图片进行了标注,建立了容量达7.85 GB的C57BL/6J小鼠主要器官的组织学数据库。结论构建可查询的C57BL/6J小鼠主要器官的形态学数据库,是研究疾病模型小鼠方便快捷的对照图库。 |
| 关 键 词： | C57BL/6J小鼠 组织学 切片 数据库 |
Establishing a digital histology library of the main organ of the C57BL/6J mice |
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| DENG Yu-jiang,CHEN Jun-ying,QIU Quan-guang,WANG Yong,WEI Hong. Establishing a digital histology library of the main organ of the C57BL/6J mice[J]. Journal of Regional Anatomy and Operative Surgery, 2010, 19(3): 166-167 | |
| Authors: | DENG Yu-jiang CHEN Jun-ying QIU Quan-guang WANG Yong WEI Hong |
| Affiliation: | Life Science Institute;Southwest University;Chongqing 400715;China |
| Abstract: | Objective To establish a digital histology library of the main organ of the C57BL/6J mice.Methods Twenty C57BL/6J mice(male 10,female 10) were anesthetized deeply,totally 45 organs and tissues were taken and fixed by 10% formalin,including heart,liver,spleen,kidney,stomach,etc.embedded by paraffin and sectioned,stained with haematoxylin and eosin,and observation under microscope.Then the images were marked in computer.The histology library was established by SQLWEVER2000 database management system,C# progra... |
| Keywords: | C57BL/ 6J mice histology section digital library |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |