小鼠耳蜗毛细胞体外培养研究方法

目的建立耳蜗Corti器体外培养模型.方法取刚出生2～3天小鼠耳蜗,采用鼠尾胶表面平铺培养并结合荧光染色方法观察耳蜗毛细胞的生长情况.结果耳蜗基底膜经1～5天培养,内、外毛细胞和支持细胞生长良好,排列有序,无任何衰亡和缺损.结论耳蜗Corti器可以在体外培养环境存活一定时间并健康生长.

Technological study on the in vitro culturing of corti''''s organ from mouse cochlea

Objective To establish a practical method for mouse Corti's organ culturing in vitro. Methods Surface procedures were taken to culture Corti's organ from mouse cochlea and influenscence was used to stain the cells in Corti's organ for observing their growing status. Results Following a culturing period lasted for 1 to 5 days,the inner and outer hair cells and supporting cells were all in an ideal growing status in a well arranged order without marked defect or loss. Conclusion The cells in Corti's organ can survival for a certain period of time in culturing condition in vitro with well growing status.