兔骨髓干细胞和脂肪干细胞体外成骨能力的比较

目的:评价两种不同来源的间充质干细胞,即骨髓干细胞(BMSC)和脂肪干细胞(ADSC)的体外成骨能力,为其将来应用于细胞治疗和组织工程研究提供一定的理论依据。方法:分别取同一只兔的髂骨骨髓和腹股沟脂肪作为BMSC和ADSC的来源,分离培养后比较成骨、成脂分化潜能,细胞表面抗原标记物;成骨诱导后检测碱性磷酸酶(ALP)活性,RT-PCR分析其成骨相关基因的表达情况。结果:BMSC和ADSC都具有向成骨、成脂分化的潜能;在成骨诱导分化后,BMSC有较高的ALP活性和较多的钙结节形成。RT-PCR结果显示:BMSC表达较高的BMP-2、OCN和OPN等成骨基因。结论:BMSC和ADSC都具有向成骨、成脂分化的潜能,ADSC有较好的成脂分化能力,BMSC有较好的成骨分化能力;两者都可以作为组织工程的种子细胞。

Osteogenic Potential of Bone Marrow vs Adipose Tissue Mesenchymal Stem Cells

Objective: This study was designed to evaluate the osteogenic capacity of rabbit mesenchymal stem cells both isolated from bone marrow(BMSCs) and adipose tissue(ADSCs) in vitro.Methods: BMSCS and ADSCS were obtained from iliac bone marrow and inguinal fat tissue respectively of the same animal.BMSCs and ADSCs were cultured in the medium and their third-passage cells were used for experiments.We compared their multi-differentiation potential, and cell surface antigen markers.Alkaline phosphatase(ALP) activity was assayed as well as to assess gene expression by RT-PCR.Results: BMSCs and ADSCs both have multilineage differentiation potentials.BMSCs expressed highest ALP activity and more calcium nodule formation after osteogenic differentiation.RT-PCR results showed that BMSCs also express higher osteogenic genes like BMP-2,OCN and OPN.Conclusion: BMSCs and ADSCs both have multilineage differentiation potentials;but compared to the ADSCs which expressed more adipogenic differentiation capacity,the BMSCs osteogenic differentiation capacity is more better.Both of them can be used as seed cells for tissue engineering.