母亲外周血单个核细胞HBV感染对围生儿的影响

目的探讨母亲HBV感染的外周血单个核细胞在HBV宫内感染中的作用。方法用套式多聚酶链反应(nPCR)法分别检测41例HBsAg阳性、12例HBsAg阴性而其他标志物阳性和16例乙肝血清标志物全部阴性的孕产妇及其新生儿血清和外周血单个核细胞中的HBVDNA。结果HBsAg阳性产妇外周血单个核细胞中的HBVDNA阳性率为31.7%(13/41);外周血单个核细胞感染率与血清HBeAg和HBVDNA的状态及水平无关(P>0.05);新生儿外周血单个核细胞HBVDNA阳性率在母亲血清和外周血单个核细胞HBVDNA均阳性组显著高于仅血清阳性组;母亲外周血单个核细胞HBVDNA阳性组显著高于阴性组(P<0.05);1例母亲血清HBVDNA阴性而外周血单个核细胞HBVDNA阳性,其新生儿血清中检出HBVDNA。结论母亲HBV感染的外周血单个核细胞有可能作为载体导致HBV宫内感染。

The influence of HBV infection in maternal PBMC on the perineonate

Objective To investigate role of HBV infection in maternal PBMC in intrauterine transmission. Methods 41pregnant women with positive HBsAg and 12 with negative HBsAg but with other positive HBV markers, and their infants were included in study group, 16 pregnant women with negative HBV and their infants were in the control group. HBV DNA in serum and PBMC of both mother and the newborn were detected respectively by nested polymerase chain reaction (n-PCR). Results The positive rate of HBV DNA in PBMC of pregnant women with positive HBsAg was 31.7%(13/41), and the HBV infection rate in PBMC was not associated with HBeAg and HBVDNA status and level in serum(P>0.05); The positive rate of HBV DNA in PBMC of infants was significantly higher in the group of both positive serum and PBMC HBV DNA than that in the group of maternal positive serum HBV DNA only (P<0.05) and the positive HBV DNA in maternal PBMC of women with positive serum HBV DNA markedly higher than that of women with negative serum HBV DNA(P<0.05); HBV DNA was detected in one of newborn's serum while his mother had HBV DNA in PBMC only. Conclusion Maternal HBV infected PBMC could be a carrier to induce intrauterine HBV infection.