| 高效液相色谱法测定甲硫氨酸维B_1注射液的含量 |
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| 引用本文： | 纪宇,刁勇,王晨霞.高效液相色谱法测定甲硫氨酸维B_1注射液的含量[J].中国生化药物杂志,2005,26(4):228-229. |
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| 作者姓名： | 纪宇 刁勇 王晨霞 |
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| 作者单位： | 1. 江苏省药品检验所,江苏南京210008
2. 南京蓝本科技有限公司,江苏南京210029 |
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| 摘 要： | 目的建立5μm高效液相色谱法同时测定甲硫氨酸维B1注射液中两组分的含量。方法用KromasilC18柱(250mm×4.6mm,5μm),流动相为1mmol/L的己烷磺酸钠溶液(含0.2%三乙胺,用50%H3PO4溶液调节pH5.0)-甲醇(85∶15),流速为1.0ml/min,检测波长为228nm。结果甲硫氨酸和维生素B1分别在80~723μg/ml与8.4~75.6μg/ml的浓度范围内呈良好的线性关系(r=0.9998与0.9999),两者的平均回收率RSD分别为(99.3±0.5)%与(99.8±0.7)%(n=9)。结论此法快速简便,有较好的精密度和准确度。
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| 关 键 词： | 高效液相色谱法 甲硫氨酸 维生素B1 含量测定 |
| 文章编号： | 1005-1678（2005）04-0228-02 |
| 收稿时间： | 2005-02-05 |
| 修稿时间： | 2005-04-14 |
HPLC determination of methionine and Vitamin B1 injection |
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| Ji Yu,DIAO yong,WANG Chen-xia.HPLC determination of methionine and Vitamin B1 injection[J].Chinese Journal of Biochemical Pharmaceutics,2005,26(4):228-229. |
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| Authors: | Ji Yu DIAO yong WANG Chen-xia |
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| Institution: | JI Yu~1,DIAO Yong~2,WANG Chen-xia~2 |
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| Abstract: | PurposeTo establish a method for simultaneous determination of methionine and Vitamin B_1 in injection by RP-HPLC.Methods10 μl of test solution was injected and separated on a Kromasil C_(18) column(250 mm×4.6 mm,5 μm)with 1 mmol/L sodium hexanesulfonate (containing 0.2% TEA, adjusted to pH (5.0) with 50% phosphoric acid)-methanol (85∶15) as mobile phase and UV detection at 228 nm. The flow rate was 1.0 ml/min.ResultsBoth methionine and Vitamin B_1 showed good linearity (r=0.999 8 and (0.999 9)) in the range of 80～723 μg/ml and 8.4～75.6 μg/ml respectively. The average recoveries were (99.3±(0.5))% and (99.8±0.7)%(n=9)respectively.ConclusionThis method was rapid, simple and accurate. |
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| Keywords: | HPLC methionine Vitamin B1 assay |
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