NQ01基因多态性与慢性苯中毒遗传易感性的研究

目的 探讨NQO1基因多态性与慢性苯中毒遗传易感性之间的关系。方法 选择100名慢性苯中毒工人为病例组及90名同期接苯但无苯中毒表现的同工种工人为对照组，应用PCR-RFLP方法判定NQ01基因型。结果携带NQ01 C609TT／T基因型(纯合突变型)个体发生苯中毒的危险性是具有C／T基因型(杂合型)和C／C基因型(野生型)个体的2．82倍(95％ CI：1．42～5．58)，是具有C／C基因型(野生型)个体的2．94倍(95％ CI:1．25-6．90)；并存在携带NQO1 C609T T／T基因型(纯合突变型)个体发生苯中毒的危险性高于携带NQ01 C609T C／T基因型(杂合型)个体、更高于C／C基因型(野生型)个体的趋势(X^uend=6．01，P=0．014)。结论 携带NQ01 C609T纯合突变基因型(T／T)个体接苯时发生苯中毒的危险性增高，考虑此基因可作为易感性生物标志物，用于苯作业工人上岗前的筛检。

Study on genetic polymorphism of NQO1 and susceptibility to benzene poisoning

OBJECTIVE: To explore the relationship between genetic polymorphism of NQO1 and susceptibility to benzene poisoning (BP). METHODS: The genetic polymorphism of NQO1 for 100 patients with benzene poisoning and 90 workers exposed to benzene who were engaged in the same working time and job title as patients with benzene poisoning were detected by PCR-RFLP. RESULTS: There was a 2.82-fold (95% CI: 1.42-5.58 ) increased risk of BP in the subjects with NQO1 C609T mutation genotype (T/T) compared with those carrying heterozygous (C/T) and wild type (C/C), and there was a 2.94-fold (95% CI: 1.25-6.90) increased risk of BP in the subjects with NQO1 C609T mutation genotype (T/T) compared with those carrying wild type (C/C) . There was a trend that the risk of BP in subjects with NQO1 C609T mutation genotype (T/T) was higher than those carrying heterozygous (C/T) and even higher than those carrying wild type (C/C) (chi2(trend) = 6.01, P = 0.014). CONCLUSION: The subjects with NQO1 C609T mutation genotype (T/T) were more susceptive to benzene and NQO1 is as a biomarker to assessment the risk of benzene poisoning for individual.