柱前衍生-超高效液相色谱法测定人血清中丙戊酸钠的浓度

目的建立用于测定人血清中丙戊酸钠浓度的超高效液相色谱法。方法采用超高效液相色谱法，色谱柱为AC—QUITYUPLC@BEHC18（50mm×2．1mm，1．7μm），柱温4J0℃，检测波长248nm，流动相：甲醇-水（60：40）（0min）→甲醇-水（80：20）（4min）→甲醇-水（100：0）（5min）→甲醇-水（60：40）（8min），流速0．5mL·min^-1。结果血清质量浓度在16．632～166．320mg·L^-1内线性良好。回归方程：Y=0．019x＋0．0684，r=0．9999。在低、中、高质量浓度下的回收率分别为98．473％、98．508％、94．606％，日内精密度RSD为0．290％-1．614％（n=5），日间精密度RSD为0．628％-2．522％（n=5）。结论本法灵敏、准确、快速、专属性强，适用于丙戊酸钠的血药浓度监测。

Pre-column derivatization- ultra-high performance liquid chromatography for the determination of sodium valproate concentration in human serum

AIM To establish an ultra-high performance liquid chromatography （UPLC） method to determine sodium valproate concentration in human serum. METHODS By ultra-high performance liquid chromatography, the AC- QUITY UPLC @BEH C18（50 mm ×2.1mm,1.7μm） column was used, column temperature was 40℃, detection wave- length was 248 nm, mobile phase was methanol - water （60：40） （0 min） →methanol - water （80：20） （4 min）→ methanol- water （100：0） （5 min）→ methanol - water （60：40） （8 min） and flow rate was 0.5 mL·L^-1. RESULTS There was good linearity within the range of 16. 632 - 166. 320 mg·L^-1. The regression equation was y = 0.019x ＋ 0. 068 4, r = 0. 999 9. In the low, medium and high concentrations recoveries were 98.473 %, 98. 508%, 94.606%, re- spectively. The intra-day precision was 0. 290% - 1. 614% （ n = 5）, and the inter-day precision was 0. 628% - 2.522% （ n = 5）. CONCLUSION The method is sensitive, accurate, rapid and specific, and suitable for sodium val- proate blood concentration monitoring.