ABCG2在白血病细胞中的表达及其意义

目的探讨三磷酸腺苷(ATP)结合转运蛋白G超家族成员2(ABCG2)在白血病细胞膜上的表达规律及其意义.方法应用CD45SSC参数设门多色流式细胞术分析61例白血病患者白血病细胞膜ABCG2及其他白血病细胞相关抗原的表达情况.结果61例急慢性白血病患者的白血病细胞ABCG2表达阳性率为36.07%.ABCG2表达阳性率,急性白血病(AL)患者[34.48%(20/58)]与慢性白血病(CL)患者[66.67%(2/3)];儿童患者[26.09%(6/23)]与成人患者[42.11%(16/38)]差别均无显著性意义(均P＞0.05);急性髓细胞性白血病(AML)患者[46.67%(14/30)]高于急性淋巴细胞性白血病(ALL)患者[16.67%(4/24)(P＜0.05)];AML患儿[55.56%(5/9)]也高于ALL患儿[7.14%(1/14)(P＜0.05)].ABCG2与另一耐药蛋白P糖蛋白(P-gp)及其他细胞表面抗原的表达均无关(r=0.152、-0.075、-0.063、0.026、-0.149,均P＞0.05),与髓系标志CD13的表达有关(r=0.307,P＜0.05).结论ABCG2在AML患儿中的表达较高,其高表达可能导致AML患儿临床耐药.ABCG2和P-gp可能参与了不同的白血病耐药机制.

Analysis of ABCG2 expression on leukemia cells and its significance

Objective To investigate the expression of ATP-binding cassette superfamily G member 2 (ABCG2) on leukemia cells and its significance. Methods Blood samples from 61 patients with leukemia were analyzed by CD45/SSC double parameters and multi-color flow cytometry to determine the expression of ABCG2 and other leukocyte differentiation antigens. Results The total positive rate of ABCG2 in 61 patients was 36.07%. ABCG2 expression was not significantly different between acute leukemia (AL) [34.48% (20/58)] and chronic leukemia (CL) [66.67%(2/3)]; ABCG2 expression in children [26.09%(6/23)] was not different from that in adult leukemia [42.11%(16/38)](P>0.05).The positive rate of ABCG2 in AML [46.67%(14/30)]was significantly higher than that in ALL [16.67%(4/24)] (P=0.02). In children AML ABCG2 expression [55.56%(5/9)] was also higher than that in ALL [7.14%(1/14)] (P=0.018). A significant association was found between ABCG2 and a myeloid related antigen CD13 (P=0.018), while no significant correlation of ABCG2 with P-glycoprotein (P-gp) and other leukocyte differentiation antigens was found. Conclusion ABCG2 expression in AML was significantly higher than that in ALL, which suggests ABCG2 may be associated with clinical resistant disease in AML.