| Differentiation of Helicobacter pylori isolates by polymerase chain reaction—restriction fragment length polymorphism |
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| 引用本文： | 施理,孙勇,等.Differentiation of Helicobacter pylori isolates by polymerase chain reaction—restriction fragment length polymorphism[J].中国人民解放军军医大学学报,2002,17(1):14-16. |
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| 作者姓名： | 施理 孙勇 |
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| 作者单位： | [1]CenterforInfectiousDiseasesofChinesePLA,458HospitalofChinesePLA,Guangzhou510602,China [2]InstituteofDigestiveDiseaseofChinesePLA,NanfangHospital,FirstMilitaryMedicalUniversity,Guang |
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| 基金项目： | Supported by National Natural Science Foundation of China (No. 39570334) |
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| 摘 要： | Objective: To investigate the association between the diversity of urease gene and urease activity of clinical isolates of Helicobacter pylori (H. pylori). Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of urease gene and rapid urease activity test were used to study the urease activity of different clinical isolates of H. pylori. Results: H. pylori clinical isolates were divided into 4 types according to their PCR-RFLP results of urease gene and urease activity. Type Ⅰ , possessing strong urease activity (0. 11) and presented 1 fragment of 1. 7 kb by PCR-RFLP, had close relations with gastric ulcer; type Ⅱ , with the weakest urease activity (0. 07) and 2 fragments (1. 3 and 0. 4 kb respectively) , was associated with duodenal bulb ulcer; type Ⅲ , with the strongest urease activity (0. 12) and 2 fragments (0. 4 and 0. 17 kb) with or without 1 fragment (0. 23 or 0. 37 kb) , was responsible for gastritis; type Ⅳ , with weak urease activity (0. 09) and 2 fragments
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| 关 键 词： | 幽门螺旋杆菌 PCR 限制片段长度多态性 株系多样化 |
Differentiation of Helicobacter pylori isolates by polymerase chain reaction-restriction fragment length polymorphism |
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| SHI Li,SUN Yong,ZHANG Ya-li,ZHANG Zhen-Shu,ZHOU Dian-yuan.Differentiation of Helicobacter pylori isolates by polymerase chain reaction-restriction fragment length polymorphism[J].Journal of Medical Colleges of PLA(China),2002,17(1):14-16. |
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| Authors: | SHI Li SUN Yong ZHANG Ya-li ZHANG Zhen-Shu ZHOU Dian-yuan |
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| Abstract: | Objective: To investigate the association between the diversity of urease gene and urease activity of clinical isolates of Helicobacter pylori (H. pylori). Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of urease gene and rapid urease activity test were used to study the urease activity of different clinical isolates of H. pylori. Results: H. pylori clinical isolates were divided into 4types according to their PCR-RFLP results of urease gene and urease activity. Type I , possessing strong urease activity (0. 11) and presented 1 fragment of 1.7 kb by PCR-RFLP, had close relations with gastric ulcer; type Ⅱ , with the weakest urease activity (0. 07) and 2 fragments (1.3 and 0. 4 kb respectively), was associated with duodenal bulb ulcer; type Ⅱ , with the strongest urease activity (0. 12) and 2 fragments (0. 4and 0. 17 kb) with or without 1 fragment (0. 23 or 0. 37 kb) , was responsible for gastritis; type Ⅳ, with weak urease activity (0. 09) and 2 fragments (1.5 and 0. 2 kb), was shown to be related to both gastric and duodenal bulb ulcers. Conclusion: The diversity of urease gene decides different urease activities of different clinical isolates of H. pylori, hence the different possibilities of pathogenesis due to this bacteria. |
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| Keywords: | Helicobacter pylori polymerase chain reaction-restriction fragment length polymorphism strain diversity |
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