骨形态发生蛋白2基因治疗与缓释载体修复骨缺损的比较研究

目的]比较骨形态发生蛋白2（BMP-2）基因治疗与生长因子缓释方法修复节段性骨缺损效果。方法]于兔双侧桡骨中段造成1．5cm骨缺损，采用4种方法修复：A组植入转基因骨髓间质干细胞（MSCs）与PLA／PCL（聚乳酸／聚己内酯）支架的复合物；B组植入单纯MSCs与含重组BMP-2的PLA／PCL缓释载体的复合物；C组植入单纯MSCs与PLA／PCL复合物；D组植入单纯PLA／PCL。术后4、8、12周行X线、组织学、生物力学和骨密度等检测，结果]A组体内植入4周后，成骨细胞和间质细胞呈BMP-2强阳性表达；其成骨速度及成骨质量均明显优于B组，12周时骨缺损完全修复、C组成骨能力较弱，而D组则无新骨形成，残留骨缺损。结论]BMP-2基因治疗是修复节段性骨缺损的好方法。

Comparison between gene therapy and gradual release carrier for bone morphogenetic protein-2 in repairing bone defects

Objective]To compare the effects between gene therapy and gradual release carrier for bone morphogenetic protein-2(BMP-2)in repairing bone defects.Method]Bone defects of 15 mm were created on the bilateral radius in rabbits and treated with four kinds of implantations,ie,composition of transgeneie MSCs and PLA/PCL(Group A),composition of MSCs and gradual release carrier for BMP-2(Group B),composition of MSCs and PLA/PCL(Group C),and PLA/PCL alone(Group D).After 4,8,and 12 weeks from the operations,X-ray,histological examination,biomechanics analysis,and bone density measurement were conducted.Result]After 4 weeks from the operations,in Group A both osteoblasts and mesenchymal cells displayed strongly positive expression of BMP-2;the speed and quality of bone formation in Group A were much better than those in Group B.After 12 weeks form the operations,bone defects were completely repaired in Group A.Conclusion]BMP-2 gene therapy is really a good method to repair segmental bone defects.