脑梗塞患者血浆抑制性氨基酸变化及兴奋性氨基酸的关系

目的目前对兴奋性氨基酸（EAA）和抑制性氨基酸（IAA）的临床研究较少，从临床方面探讨EAA，IAA在脑梗塞发展过程中的作用可为今后临床深人研究提供一定的参考资料。方法应用高效液相色谱法对44例动脉粥样硬化性血栓性脑梗塞病人急性期和病后2周血浆EAA和IAA的含量进行测定，进行病人自身前后对照并与30例正常人对比。结果脑梗塞病人急性或血浆谷氨酸（Glu）、天门冬氨酸（Asp）、牛磷酸（Tau）含量明显高于正常对照组（P＜0.01）；病后2周其血浆含量有所下降，其中Glu含量与急性期相比有显著性差异（P＜0．01），但三者均未恢复正常，与正常对照组相比仍有显著性差异（P＜0．01）；血浆丙氨酸（Ala）、甘氨酸（Gly）在脑梗塞急性期及病后2周与正常对照组相比均无显著性差异（P＞0．05）。结论Glu、Asp作为损伤因素参与了脑梗塞的发生发展过程，研究EAA在脑梗塞中的作用时应同时观察IAA的相应变化．兴奋性毒性指数的概念值得进一步研究。测定血浆Glu的含量可考虑作为估计脑梗塞病程的一项生化指标。

Plasma concentrations of excitatory and inhibitory amino acid in cerebral infarction and their relationshiops

Objection Our study was to discuss the effect of EAA and IAA in cerebral infartion from clinic and to afford reference for further investigation. Metheod Using high performance liquid chromatography (HPLC ), we determined the EAA including glutamate (Glu) and sspartate (Asp ), and the IAA including taurine (Tau) glycine (Gly) and alanine (Ala) concentrations in the plasma of 44 patients with arteriosclerotic thrombotic cerebral infarction in its acute phase (A group) and 30 cases among the 44 patients 2 weeks after the onset (B group) and of 30 control subjects (C group). Result The data of the two Patient groups were compared with each other and with the control group resistively. The results showed that plasma concentrations of Glu. Asp. Tau of the patient group of acute phase were aignificantly higher than that of the control group (P. < 0. 01 ). They decrease 2 weeks later but significantly differed from the control group(P < 0. 01 ), although the plasma concentration of Gin was significantly different from that of the acute phase (P < 0. 01). The plasma concentrations of Ala and Gly had no significant differences with the control group in both acute phase and 2 weeks after the onset phase (P > 0. 05 ). Conclusion Our result support the excitatory activity of EAA and Gin Asp may take part in the developing and progressing of cerebral infarction. The role of IAA and the "excitatory index" in cerebral infarction should be considered simutaneously. The plasma concentration of Glu can be considered to be an important biochemical indicator to estimate the progression of cerebral infarction.