直链烷基苯磺酸钠对小鼠皮肤组织的氧化应激损伤 及大豆异黄酮的拮抗作用研究

目的:探讨不同剂量直链烷基苯磺酸钠(LAS)对小鼠皮肤组织氧化应激的影响及大豆异黄酮的拮抗作用。方法:将40只昆明种雄性小鼠随机分成4组,每组10只,分别为正常对照组、LAS低剂量组(LAS 150 mg/L)、中剂量组(LAS 300 mg/L)和高剂量组(LAS 600 mg/L)。用蒸馏水及不同剂量的LAS涂抹小鼠的背部剃毛处,连续涂抹60 d。将50只小鼠随机分为5组,分别为正常对照组、LAS氧化应激损伤组(LAS 300 mg/ L)及3个拮抗组(分别用50、100及150 mg/L大豆异黄酮涂抹,再用300 mg/L LAS涂抹),每天1次,连续涂抹60 d。取涂抹部位皮肤,剪碎制匀浆,检测其中丙二醛(MDA)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性。结果:小鼠皮肤经不同剂量LAS处理后,MDA的含量均明显高于对照组(P<0.01),LAS高剂量组的MDA含量均明显高于低剂量组和中剂量组(P<0.01);LAS中、高剂量组 SOD和GSH-Px的活性均明显低于对照组(P<0.01),且随LAS剂量增加,酶的活性逐渐降低(P<0.01)。大豆异黄酮干预后,各拮抗组MDA含量均低于LAS氧化应激损伤组(P<0.05~P<0.01),但均高于对照组(P<0.01);除拮抗组1与LAS氧化应激损伤组GSH-Px活性差异无统计学意义(P >0.05)外,各拮抗组SOD和GSH-Px活性均高于LAS氧化应激损伤组(P<0.01),但均低于对照组(P<0.05~0.01);随异大豆黄酮浓度的增加,拮抗效果增加。结论:LAS可导致小鼠皮肤组织氧化应激损伤,大豆异黄酮在一定剂量范围内可以抑制这种应激损伤。

Effect of linear alkylbenzenesulfonate on the oxidative stress damage of mouse skin and antagonism of isoflavone

Objective:To investigate the effects of linear alkylbenzenesulfonate(LAS) om the oxidative stress damage of mouse skin and antagonism of isoflavone. Methods:Forty healthy male Kunming mice were randomly divided into the control group,low-dosage LAS group(150 mg/ L),middle-dosage LAS group(300 mg/ L) and high-dosage LAS group(600 mg/ L)(10 mice each group). The distilled water and different dosage LAS were smeared on the back skin of mice once a day for 60 days. Fifty mice were randomly divided into the control group,LAS oxidative stress damage model group(skin smeared with 300 mg/ L of LAS) and three antagonistic groups( skin smeared with 300 mg/ L LAS after smearing 50, 100 and 150 mg/ L of isoflavone, once a day for 60 days). The content of malondialdehyde(MDA)and activities of super oxidase dimutase(SOD) and glutathione peroxidase(GSH-Px) in all mice skin were detected. Results:The content of MDA in mice skin smeared with LAS was remarkably higher than that in control group(P < 0. 01), and the contents of MDA in high-dosage LAS group were significantly higher than that in low- and middle-dosage LAS groups(P <0. 01). The activities of SOD and GSH-Px in middle- and high- dosage LAS group were obviously lower than those in control group(P <0. 01),which decreased with the dosage increasing of LAS( P < 0. 01). After isoflavone intervention,the contents of MDA in all antagonistic groups were lower than that in LAS damage group,and higher than that in control group(P < 0. 05 to P < 0. 01). Except the antagonistic group 1,the differences of the GSH-Px activity between other antagonistic groups and LAS damage groups were not statistically significant ( P > 0. 05), the activities of SOD and GSH-Px in antagonistic groups were higher than those in LAS damage groups,and lower than those in control group(P < 0. 05 to P < 0. 01 ). The antagonistic effects increased with the isoflavone concentration increasing. Conclusions:LAS can lead to the oxidative stree damage of mouse skin,and the isoflavone can restrain the damage within a certain range of dosage.