The utility of multiplex polymerase chain reaction for diagnosis of infectious diarrhoea in a tropical country |
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| Affiliation: | 1. Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences (SGPGIMS), Raebareli Road, Lucknow, 226014, India;2. Department of Nephrology, Sanjay Gandhi Postgraduate Institute of Medical Sciences (SGPGIMS), Raebareli Road, Lucknow, 226014, India;3. Department of Gastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences (SGPGIMS), Raebareli Road, Lucknow, 226014, India;1. Department of Clinical Microbiology & Immunology, Sir Ganga Ram Hospital, Rajinder Nagar, New Delhi, 110060, India;2. Critical Care & Emergency Medicine, Sir Ganga Ram Hospital, Rajinder Nagar, New Delhi, 110060, India;3. Department of Internal Medicine, Sir Ganga Ram Hospital, Rajinder Nagar, New Delhi, 110060, India;1. Department of ENT, Christian Medical College, Vellore, 632004, India;2. Department of Microbiology, Christian Medical College, Vellore, 632004, India;3. Department of Infectious Diseases, Christian Medical College, Vellore, 632004, India;1. Department of Nephrology, Kokilaben Dhirubhai Ambani Hospital and Medical Research Institute, Mumbai, India;2. Department of Infectious Disease, Kokilaben Dhirubhai Ambani Hospital and Medical Research Institute, Mumbai, India;3. Department of Critical Care, Kokilaben Dhirubhai Ambani Hospital and Medical Research Institute, Mumbai, India;4. Department of Microbiology, Kokilaben Dhirubhai Ambani Hospital and Medical Research Institute, Mumbai, India;1. Enterovirus and Viral Gastroenteritis Unit, National Centre for Microbiology (CNM), Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo km 2, Madrid, Spain;2. Microbiology Department, Hospital Universitario Puerta de Hierro, Madrid, Spain;3. MATERLAB, S.L., Madrid, Spain |
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| Abstract: | ![]()
PurposeThe etiology of infective diarrheaoften remains undiagnosed. We studied the role of multiplex polymerase chain reaction (PCR) for detection of etiological agents of diarrhoea.MethodsFast track diagnostics (FTD)gastroenteritis panel for bacterial and viral pathogens was used to test stool samples from patients with diarrhoea.ResultsStool samples from 276 patients (138 immunocompetent and 138 immunocompromised) with diarrhoea and 138 healthy controls were tested. Bacterial culture was positive in 5 samples. Following agents were isolated: Shigella sonnei(2), Shigella dysentriae(1), SalmonellaParatyphi B(1) and Vibrio cholerae (1). Multiplex PCR panel did not include Vibrio cholerae in its panel. A total of 65 target pathogens were identified in 60/276 (21.7%) patients by multiplex PCR. 28/65(41.1%) and 37/65 (56.9%) were bacterial and viral agents respectively. Identified bacteria were Shigella(20), Salmonella(3), Campylobacter(4) and Clostridium difficile(1). Viral targets identified were Norovirus GII (28), Adenovirus(4), Astrovirus(3) and Sapovirus(2). All the controls were negative for enteropathogens by conventional methods and multiplex PCR.ConclusionsOur detection rates increased from 1.8% (5/276)by conventional methods to 21.7% (60/276)by multiplex PCR, which included both bacterial as well as viral targets. |
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| Keywords: | Gastroenteritis Multiplex PCR Molecular diagnosis |
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