| 高效液相色谱法测定香薷药材中香芹酚和百里香酚的含量 |
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| 引用本文： | 吉力,汪芳,刘元艳,仝燕,李先端,冯学峰,黄璐琦,周国平.高效液相色谱法测定香薷药材中香芹酚和百里香酚的含量[J].中国中药杂志,2004,29(11):1030-1032. |
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| 作者姓名： | 吉力 汪芳 刘元艳 仝燕 李先端 冯学峰 黄璐琦 周国平 |
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| 作者单位： | 1. 中国中医研究院,中药研究所,北京,100700
2. 江西省药品检验所,江西,南昌,330000 |
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| 基金项目： | 中央级科研院所科技基础性工作专项基金 |
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| 摘 要： | 目的 :建立香薷药材中香芹酚和百里香酚的含量测定方法。方法 :用高效液相色谱法 ,95 %乙醇超声提取 ,色谱柱InertsilODS-3,流动相甲醇 水 冰醋酸 (60∶40∶2 ) ,检测波长 274nm。结果 :香芹酚在 023～215μg (r=0.9999) ,百里香酚在 039～236μg (r =0.9999)有良好的线性关系 ;平均回收率分别为香芹酚 99.9% (RSD 1.4 % ) ,百里香酚 98.6 % (RSD 1.3% ) ,重复性的RSD分别为香芹酚 1.1% ,百里香酚 1.6 %。结论 :该方法可靠、便捷 ,可以作为控制香薷药材质量的方法。
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| 关 键 词： | 石香薷 香芹酚 百里香酚 高效液相色谱法 |
| 文章编号： | 1001-5302(2004)11-1030-03 |
| 收稿时间： | 2004/4/16 0:00:00 |
Determination of carvacrol and thymol in Mosla chinensis by HPLC |
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| JI Li;WANG Fang;LIU Yuan-yan;TONG Yan;LI Xian- duan;FENG Xue-feng;HUANG Lu-qi;ZHOU Guo-ping.Determination of carvacrol and thymol in Mosla chinensis by HPLC[J].China Journal of Chinese Materia Medica,2004,29(11):1030-1032. |
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| Authors: | JI Li;WANG Fang;LIU Yuan-yan;TONG Yan;LI Xian- duan;FENG Xue-feng;HUANG Lu-qi;ZHOU Guo-ping |
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| Institution: | Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China. |
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| Abstract: | OBJECTIVE: To establish a quantitative method of determination of carvacrol and thymol in Mosla chinensis. METHOD: The sample was extracted with 95% ethanol, ODS column was used with methanol-water-acetic acid (60:40:2) as mobile phase. The detection wavelength was set at 274 nm. RESULT: The linearities of carvacrol and thymol were respectively in the range of 0.23-2.15 microg (r = 0.9999) and 0.39-2.36 microg (r = 0.9999); the average recoveries were 99.9% (RSD 1.4%) and 98.6% (RSD 1.3%); the RSD of repeatability were 1.1% and 1.6%. CONCLUSION: The method is reliable, and can be used for quality control of M. chinensis. |
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| Keywords: | Mosla chinensis carvacrol thymol HPLC |
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