| 抗HLA-B27单克隆抗体的制备鉴定及初步应用 |
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| 引用本文： | 吴福国,论宁,韩晓琳,马玉玲,张向阳,孔庆胜,李淑玲,郭子林,刘玉庆,朱立平. 抗HLA-B27单克隆抗体的制备鉴定及初步应用[J]. 济宁医学院学报, 2002, 25(1): 13-14 |
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| 作者姓名： | 吴福国 论宁 韩晓琳 马玉玲 张向阳 孔庆胜 李淑玲 郭子林 刘玉庆 朱立平 |
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| 作者单位： | 济宁医学院生化教研室;中国协和医科大学 |
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| 基金项目： | 山东省卫生厅和教育厅资助课题 |
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| 摘 要： | 目的：制备HLA－B27单克隆抗体。方法：用HLA－B27抗原免疫小鼠,取脾细胞与骨髓瘤细胞融合制备杂交瘤细胞,用ELISA法筛选分泌该单抗的细胞株。结果：建立了分泌该单抗的细胞系3B3和4F2,抗体均为IgG2b,3B3细胞分泌的单抗只与B27反应,而4F2细胞分泌的单抗与B7有交叉反应。结论：建立了高特异性分泌抗B27单抗的细胞株,具有潜在的应用价值。
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| 关 键 词： | 单克隆抗体 人类白细胞抗原B27 强直性脊柱炎 |
PREPARATION AND CHARACTERIZATION OF HLA- B27 MONOCLONAL ANTIBODY |
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| Wu Fuguo,Lun Ning,Han Xiaolin,et al. PREPARATION AND CHARACTERIZATION OF HLA- B27 MONOCLONAL ANTIBODY[J]. Jounal of Jinning Medical College, 2002, 25(1): 13-14 |
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| Authors: | Wu Fuguo Lun Ning Han Xiaolin et al |
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| Abstract: | Objective To preparation HLA-B27 monoclonal antibody.Methods HLA-B27mAb was derived from fusion of mouse SP2/0 myeloma cells with spleen cells from BALB/c mouse immunized with a purified HLA-B27 antigen using a conventional fusion technique. ELISA method was used for screening.Results Two clones named 3B3 and 4F2, belonging to IgG2b , were finally obtained. The specificity of these mAbs was examined by microcytotoxicity test. No cross reactivity with B7 and B22 antigens was detected in 3B3 cell lines, but there was cross reactivity between B27 mAb(from 4F2cell lines) and B7 antigen.Conclusion HLA-B27 mAb (3B3) was a highly specific monoclonal antibody. |
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| Keywords: | Monoclonal antibody HLA-B27 Ankylosing spondylitis |
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