| 电针对脊髓损伤后一氧化氮合酶表达的影响 |
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| 引用本文： | 张志英,严振国. 电针对脊髓损伤后一氧化氮合酶表达的影响[J]. 中国组织工程研究与临床康复, 2002, 6(2): 206-207 |
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| 作者姓名： | 张志英 严振国 |
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| 作者单位： | 上海中医药大学,上海,200032 |
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| 摘 要： | 目的探讨电针对脊髓损伤后一氧化氮合酶(nitricoxidesynthase,NOS)基因及其蛋白表达的影响。方法采用改良的Allen's垂击法致大鼠脊髓(T10)中度损伤,损伤后即刻进行督脉电针治疗,于术后1、4、24、48h处死动物,分别用RT-PCR法和NADPH-黄递酶组织化学染色法测定各型NOSmRNA及其蛋白的表达,并进行图像定量统计学分析。结果电针可不同程度地降低nNOSmRNA、iNOSm-RNA的表达,降低NOS活性。结论电针早期治疗可能通过降低一氧化氮(NO)的形成减轻脊髓继发性损伤,有一定的神经保护作用。
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| 关 键 词： | 电针 一氧化氮合酶 一氧化氮 脊髓损伤 |
| 文章编号： | 1671-5926(2002)02-0206-02 |
| 修稿时间： | 2001-12-20 |
The effect of electric-acupuncture on nitric oxide synthase expression in spinal cord injury |
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| ZHANG Zhi-ying,YAN Zhen-guo. The effect of electric-acupuncture on nitric oxide synthase expression in spinal cord injury[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2002, 6(2): 206-207 |
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| Authors: | ZHANG Zhi-ying YAN Zhen-guo |
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| Affiliation: | ZHANG Zhi-ying,YAN Zhen-guo.Shanghai University of Traditional Chinese Medcine and Pharmacology,Shanghai 200032,China |
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| Abstract: | Objective To study the effect of electric-acupuncture on nitric oxide synthase gene and protein expression in rat spinal cord injury. Method The rats were treated in electric-acupuncture after spinal cord injury(T10) by Allen's weight-drop (50 g-cm force) technique and killed at 1, 4, 24, 48 h after operation. Each NOSmRNA and protein of NOS were assayed by RT-PCR, NADPH-Diaphorase histochemistry and image analysis system. Result The expression of nNOSmRNA, iNOSmRNA and activity of NOS were downregulated by electric acupuncture. Conclusion Electric-acupuncture may prevent the progress of the secondary spinal cord injury by decreasing the synthesis of NO and have some protection role on neuronal function. |
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| Keywords: | Electric-acupuncture nitric oxide synthase nitric oxide spinal cord injury |
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