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| 前列舒胶囊的质量标准研究 | |
| 引用本文： | 张俊红,李赐恩,何艳青. 前列舒胶囊的质量标准研究[J]. 中国实用医药, 2011, 6(15): 24-26 |
| 作者姓名： | 张俊红 李赐恩 何艳青 |
| 作者单位： | 广州中医药大学第一附属医院,510405 |
| 摘 要： | 目的建立前列舒胶囊的质量标准。方法采用薄层色谱法对前列舒胶囊中黄柏、蒲公英、栀子、赤芍等进行定性鉴别;采用高效液相色谱法测定盐酸小檗碱的含量。色谱柱:PHENOMENEXODSC18柱(4.6×250mm,5μm);流动相:乙腈-0.1%磷酸溶液(40:60)(每100ml含十二烷基磺酸钠0.1g);检测波长:345nm;流速:1ml/min;柱温:30℃。结果盐酸小檗碱在32.6～326.0ng范围内呈良好线性关系。平均回收率为96.84%,RSD为1.30%(n=6)。结论该方法简便、准确、可靠,可作为前列舒胶囊的质量控制方法。 |
| 关 键 词： | 前列舒胶囊 质量标准 薄层色谱法 高效液相色谱法 盐酸小檗碱 |
Studies on the quality standard of qianlieshu capsule |
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| ZHANG Jun-hong,LI Ci-en,HE Yan-qing. Studies on the quality standard of qianlieshu capsule[J]. China Practical Medical, 2011, 6(15): 24-26 | |
| Authors: | ZHANG Jun-hong LI Ci-en HE Yan-qing |
| Affiliation: | ZHANG Jun-hong,LI Ci-en,HE Yan-qing.The First Affiliated Hospital of Guagnzhou University of Chinaese Medicine,Guangzhou 510450,China |
| Abstract: | Objective To establish the quality standard of Qianlieshu capsule.Methods TLC were used to distinguish Huangbai,Pugongying,Zhizi and Chishao.HPLC were used for the deterimination of Berberine.The column was PHENOMENEX ODS C18（4.6 × 250 mm,5 μm）,the mobile phase were acetonitrile0.1% phosphoric acid（40 ︰ 60）（including 0.1 g Sodium dodecyl sulfate per 100 ml） ;the detection wavelength at 345nm,flow rate at 1.0 ml/min and column temperature at 30℃ respectively.Results Berberine showed a good linear relationship in the range of 32.6 ～ 326.0ng,and the average recovery was 96.84% with RSD = 1.30%（n = 6）.Conclusion This method is simple,accurate,reliable and can be used as a quality standard for Qianlieshu capsule. |
| Keywords: | Qianlieshu capsule Quality standard TLC HPLC Berberine |
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