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| 溶瘤腺病毒SG7605-11R-P53对肝癌细胞的体外杀伤作用 | |
| 引用本文： | 于丽,陈艳,付玉华,范丽,刘辉,苏长青,钱其军. 溶瘤腺病毒SG7605-11R-P53对肝癌细胞的体外杀伤作用[J]. 中国肿瘤生物治疗杂志, 2011, 18(4): 378-382. DOI: 10.3872/j.issn.1007-385X.2011.04.007 |
| 作者姓名： | 于丽 陈艳 付玉华 范丽 刘辉 苏长青 钱其军 |
| 作者单位： | 1. 浙江理工大学生命科学学院新元医药和生物技术研究所,浙江杭州,310018 2. 第二军医大学东方肝胆外科医院基因与病毒治疗实验室,上海,200438 3. 浙江理工大学生命科学学院新元医药和生物技术研究所,浙江杭州310018;第二军医大学东方肝胆外科医院基因与病毒治疗实验室,上海200438 |
| 基金项目： | 国家新药创制重大专项课题（No. 2009ZX09102-235） |
| 摘 要： | 目的:研究携带细胞穿膜肽11R和P53的溶瘤腺病毒SG7605-11R-P53对肝癌细胞的体外杀伤作用。方法:以本课题组前期实验构建的携细胞穿膜肽11R和P53的溶瘤腺病毒SG7605-11R-P53和不携11R的溶瘤腺病毒SG7605-P53感染肝癌细胞HepG2、SMMC-7721、Hep3B、Huh7和正常成纤维细胞株BJ,Western blotting检测感染后细胞P53和11R-P53的表达情况,TCID50法检测SG7605-11R-P53和SG7605-P53在肝癌细胞中的增殖能力,MTT法检测SG7605-11R-P53对肝癌细胞及正常细胞的杀伤作用。结果:SG7605-11R-P53和SG7605-P53能在肝癌细胞中高表达P53和11R-P53蛋白。SG7605-11R-P53可在HepG2、SMMC-7721、Hep3B和Huh7细胞中大量增殖,其增殖倍数是SG7605-P53的10~100倍,但在正常BJ细胞内几乎不增殖。SG7605-11R-P53在MOI=0.1时对Hep3B细胞的杀伤率达90%,对于正常BJ细胞只有当MOI=50时才有很弱的抑制作用;SG7605-11R-P53对4种肝癌细胞杀伤作用的大小依次为Hep3B、HepG2、Huh7和SMMC-7721细胞。结论:携带细胞穿膜肽11R和P53的SG7605-11R-P53溶瘤腺病毒体外对4种肝癌细胞株均有较好的靶向杀伤作用,尤其对Hep3B细胞的杀伤作用最强。 |
| 关 键 词： | 溶瘤腺病毒 细胞穿膜肽 11R P53 肝癌 基因-病毒治疗 |
| 收稿时间： | 2011-04-15 |
| 修稿时间： | 2011-06-10 |
Cytotoxicity effect of oncolytic adenovirus SG7605-11R-P53 on hepatocellular carcinoma cells in vitro |
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| YU Li,CHEN Yan,FU Yu-hu,FAN Li,LIU Hui,SU Chang-qing and QIAN Qi-jun. Cytotoxicity effect of oncolytic adenovirus SG7605-11R-P53 on hepatocellular carcinoma cells in vitro[J]. Chinses Journal of Cancer Biotherapy, 2011, 18(4): 378-382. DOI: 10.3872/j.issn.1007-385X.2011.04.007 | |
| Authors: | YU Li CHEN Yan FU Yu-hu FAN Li LIU Hui SU Chang-qing QIAN Qi-jun |
| Affiliation: | Xinyuan Institute of Medicine and Biotechnology, Life Sciences College, Zhejiang Sci-Tech University, Hangzhou 310018, Zhejiang, China;Laboratory of Viral and Gene Therapy, Eastern Hepatobiliary Surgery Hospital, Second Miltitary Medical University, Shanghai 200438, China;Xinyuan Institute of Medicine and Biotechnology, Life Sciences College, Zhejiang Sci-Tech University, Hangzhou 310018, Zhejiang, China;Xinyuan Institute of Medicine and Biotechnology, Life Sciences College, Zhejiang Sci-Tech University, Hangzhou 310018, Zhejiang, China;Laboratory of Viral and Gene Therapy, Eastern Hepatobiliary Surgery Hospital, Second Miltitary Medical University, Shanghai 200438, China;Laboratory of Viral and Gene Therapy, Eastern Hepatobiliary Surgery Hospital, Second Miltitary Medical University, Shanghai 200438, China;Xinyuan Institute of Medicine and Biotechnology, Life Sciences College, Zhejiang Sci-Tech University, Hangzhou 310018, Zhejiang, China; Laboratory of Viral and Gene Therapy, Eastern Hepatobiliary Surgery Hospital, Second Miltitary Medical University, Shanghai 200438, China |
| Abstract: | Objective: To study the cytotoxicity of oncolytic adenovirus SG7605-11R-P53 containing cell-penetrating peptide(11R) on hepatocellular carcinoma cells Hep3B and Huh7 in vitro.Methods: Western blotting analysis was used to detect the expression levels of P53 and 11R-P53 in hepatocellular carcinoma cell lines HepG2,SMMC-7721,Hep3B and Huh7,and normal cell line BJ after SG7605-11R-P53 and SG7605-P53 infection.TCID50 assay was used to evaluate the replication ability of SG7605-11R-P53 and SG7605-P53 in hepatoce... |
| Keywords: | oncolytic adenovirus cell-penetrating peptide 11R P53 hepatocellular carcinoma cell gene-viral therapy |
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