上皮细胞增殖和凋亡在中耳胆脂瘤发病学的意义(附33例报告)

目的 :通过探讨胆脂瘤上皮 (CE)、胆脂瘤患者外耳道上皮 (CAMS)和正常健康者外耳道上皮(NAMS)的增殖和凋亡及 p5 3基因与上皮细胞增生和凋亡的关系 ,阐明增殖和凋亡异常在胆脂瘤发病中的作用。方法 :采用免疫组织化学和原位末端标记技术检测细胞增殖标记物 PCNA、Ki- 6 7和 p5 3蛋白在 33例 CE、2 5例CAMS和 10例 NAMS细胞的表达及细胞凋亡。结果 :在上述标本中均有 PCNA表达及凋亡细胞存在 ,但不同类型上皮阳性细胞数量、染色强度及分布不同。 CE存在过度增殖和凋亡 ,CAMS亦存在过度增殖。 p5 3表达与PCNA呈正相关 ,与细胞凋亡呈负相关。结论 :CE具有过度增殖和凋亡的特性 ,增殖与凋亡的紊乱与胆脂瘤形成密切相关。

Role of epithelial cell proliferation and apoptosis in human middle ear cholesteatoma

Objective:To determine the role of middle ear epithelial cell proliferation and apoptosis in the pathogenesis of human cholesteatoma.Method:33 cholesteatoma, 25 auditory meatal skin of cholesteatoma and 10 nomal human auditory meatal skin were detected by using immunohistochemistry analysis for cell proliferation with Ki 67, PCNA and PCNA antibody and TUNTEL methods for cell apoptosis. Result:PCNA expression and apoptosis cells exists different quantity in cholesteatoma, auditory metal skin of cholesteatoma and nomal human auditory meatal skin and distributes in various cell layer.There were positive correlation between p53 and PCNA as well as negative correlation between p53 and apoptosis.Conclusion:The dyregulation of proliferation and apoptosis in keratinocyte is important in pathogenesis of cholesteatoma.