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| 克隆荧光抗体染色法与金标免疫斑点法检测沙眼衣原体的比较 | |
| 引用本文： | 刘洪庆,徐秀香. 克隆荧光抗体染色法与金标免疫斑点法检测沙眼衣原体的比较[J]. 内蒙古医学杂志, 2006, 38(5): 431-432 |
| 作者姓名： | 刘洪庆 徐秀香 |
| 作者单位： | 山东省沂南县妇幼保健院检验科,山东,临沂,276300;山东省沂南县界湖镇卫生院,山东,界湖,276300 |
| 摘 要： | 目的:比较克隆荧光抗体染色法(DFA)与金标免疫斑点法(DOT)检测沙眼衣原体的优越性。方法:用克隆荧光抗体染色法与金标免疫斑点法对386例疑似患者标本进行检测,并对两种方法的检测结果进行比较分析。结果:克隆荧光抗体染色法与金标免疫斑点法对比386份标本的检测阳性率分别为28.2%和16.32%,二者结果有显著差异,P<0.05。结论:克隆荧光抗体染色法与金标免疫斑点法相比较具有专一性、特异性、灵敏度高、快捷方便等优点,对沙眼衣原体的早期诊断具有特殊意义。 |
| 关 键 词： | 沙眼衣原体 克隆荧光抗体染色法 金标免疫斑点法 |
| 文章编号： | 1004-0951（2006）05-0431-02 |
| 收稿时间： | 2004-08-04 |
| 修稿时间： | 2004-08-04 |
DAF and DOT Detection and Compare in Chamvdia Trachomatis |
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| LIU Hong-qing,XU Xiu-xiang. DAF and DOT Detection and Compare in Chamvdia Trachomatis[J]. Inner Mongolia Medical Journal, 2006, 38(5): 431-432 | |
| Authors: | LIU Hong-qing XU Xiu-xiang |
| Affiliation: | 1, Department of inspects lab of women and children hospital of Yinan county, Linyi 276300 China ; 2. Department of inspect lab hospital of Yinan county Jiehu town seat ,Jiehu 276300 China |
| Abstract: | Objective:To study the superiority significance of DFA and DOT in detection chlamydia trachomatis.Methods:386 patients specimens go on detection by DFA and DOT method.And as the detection result of two methods goes on compare and analyses.Results:386 patient specimens were identified by DFA and DOT,the positive rate was 28.2% and 16.32%.Two methods had notable discrepancy(P<0.05).Conclusions:Compared with DOT is simple sensitive and suitable for clinical application DFA for detecting chlamydia trachomatis in patient specimens is of clinical significance in early diagnosis. |
| Keywords: | Chlamydia trachomatis DFA DOT |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |