二烯丙基二硫诱导K562细胞凋亡及与G2 / M期阻滞关系研究

目的探讨二烯丙基二硫（DADS）对体外培养的人白血病细胞系K562细胞增殖、凋亡和细胞周期的影响。方法采用细胞计数法、形态学观察、MTT分析法、AO／EB染色、流式细胞术探讨DADS对K562细胞增殖、凋亡和细胞周期的影响。结果1．DADS在10-80mg-L。范围内，对K562细胞的抑制作用呈剂量一时间依赖效应。2．DADS浓度在10-80mg．L-1。时作用K562细胞24h后，凋亡率逐渐升高，差异有统计学意义（p〈0．05或P〈0．01）。3．不同浓度DADS作用于K562细胞24h后，K562细胞阻滞于G2／M期。结论DADS有抑制K562细胞增殖和促进K562细胞凋亡的作用，其作用的可能机制与k562细胞阻滞于G2／M期有关。

Diallyl disulfide induced apoptosis in K562 cells is associated with the arrest of G2/M

Objective To explore the effect of Dialllyl disulfide on the proliferation, apoptosis and cell cycle of K562. Method Cell morphology, MTY assay, AO/EB staining, flow cytometry were employed to examine DADS-induced apoptosis and growth inhibition in human Leukemia cell Line K562. Rusults 1.DADS inhibited proliferation of K562 cells from 10-80 mg.L-1 in a dose-and time-dependent manner. 2.From 10-80 mg.L-1 DADS induced apotosis of K562 cell after the treatment for 24 hours, the difference is significant （/9〈0.05 or P〈0.01）. 3.K562 cell treated with DADS significantly increased in the percentage of cells in the GE/M phase. Conclusion DADS has apparent anti-proliferation and apoptosis-indueing effects on K562cells in vitro. Arrest of G/M phase might induce apoptosis of K562 cells.