芹菜素在静水压下对人体腰椎间盘蛋白多糖合成的影响

[目的]探讨中药提取物芹菜素在静水压下对体外培养的人体腰椎间盘蛋白多糖合成的影响.[方法]32例后路腰椎间盘切除术的志愿患者中获取新鲜的32个椎间盘样品,每1例样品被切成1～2 mm3大小的碎块,与1ml培养基DMEM一同装入2.5ml的注射器中,放入培养椎间盘组织的压力装置.培养基中分别加入芹菜素(Apigenin)、一氧化氮合成酶的竞争性抑制剂NG-Monomethyl-L-arginine(简称L-NMMA)及一氧化氮的供体Sodium Nitroprusside (SNAP),在可调静水压装置中培养2h后提取上清液,采用考马斯亮蓝蛋白定量检测法测定上清液中蛋白多糖的含量.[结果]芹菜素3atm组和L- NMMA3atm组蛋白多糖合成最高,两组间相比无显著性差异(P＞0.05),与空白组相比有显著性差异(P＜0.05),SNAP 30 atm组蛋白多糖合成最低,与各组间相比均有显著性差异(P＜0.01).空白组、L-NMMA组、SNAP组和芹菜素组中3 atm下蛋白多糖合成较30 atm下蛋白多糖合成高,与之相比有显著性差异(P＜0.05).[结论]3 atm的静水压下,培养基中添加芹菜素使蛋白多糖合成明显增加.添加芹菜素也部分降低了 30atm的静水压下对蛋白多糖合成的抑制.

The effects of apigenin on proteoglycan synthesis in the human lumbar discs under hydrostatic pressure

[Objective]To investigate the effects of apigenin on proteoglycan synthesis in the human lumbar intervertebral disc in response to hydrostatic pressure.[Method]Thirty-two lumbar intervertebral disc were obtained from patients who had undergone posterior discectomy.These samples were chopped into 1-2 mm3 and incubated in a 2.5 ml’s plastic syringe with 1 ml Dulbecco’s Modified Eagle’s Medium(DMEM).Apigenin,L-NMMA and SNAP were added into the DMEM,cultured for 2 hours in the hydrostatic pressure devices,and then measured the proteoglycan content in the supernatant by means of Coomassie brilliant blue albument ration.[Result]Apigenin 3 atm group and L-NMMA 3 atm group had the highest rate of proteoglycan synthesis,there were no significant difference between the two groups(P>0.05),there was significant difference compared with control group(P<0.05)(however,they were siginificantly higher than that of control group).SNAP 30atm group had the lowest rate of proteoglycan synthesis,significant differences were found when compared with the various groups(P<0.01).In control group,L-NMMA group,SNAP group and apigenin group under the 3 atm’s hydrostatic pressure proteoglycan synthesis rates were significantly higher than under the 30 atm’s,there were significant difference compared with it(P<0.05).[Conclusion] Apigenin can increase proteoglycan synthesis ratio of the intervertebral disc under 3atm’s hydrostatic pressure,and reduce the 30atm’s hydrostatic pressure’s inhibition on proteoglycan synthesis ratio.