反相高效液相色谱法测定乌金胶囊中绿原酸的含量

目的建立反相高效液相色谱法测定乌金胶囊中绿原酸含量,并应用于该制剂质量控制。方法使用Agilent extendC₁₈柱（4.6 mm x 250 mm,5μm）,乙腈-0.1%甲酸（8:92）为流动相,流速1.0 ml/min,柱温30℃,检测波长327 nm,测定5批乌金胶囊中绿原酸含量。结果绿原酸在2.04～102μg/ml（r=0.999 9）范围内具有良好线性加样回收率104.68%,RSD=1.43%（n=6）5批乌金胶囊中绿原酸含量在55.08～83.31μg/g之间。结论该方法灵敏、准确,适用于乌金胶囊绿原酸含量洲定。

Determination of chlorogenic acid content in wujin capsule by RP-HPLC

Objective To establish a RP-HPLC method for determination of chlorogenic acid in wujing capsule. Methods Agilent extend-C18（4.6 mm ×250 mm,5 μm）column was used. The mobile phase was acetonitrile： 0.1% formic acid （8 ： 92）. The flow rate was 1.0 ml/min, and the temperature was at 35 ℃. The detection wavelength was 327 nm. Determination of chlorogenic acid in wujin capsule of 5 batches of preparations. Results The calibration curve was at the range of 2.04- 102μg/ml （ r = 0. 999 9 ） , and the average recovery was 104.68 % , and RSD was 1.43% （n = 6）. 5 batches of chlorogenic acid contents in wujin capsule were between 55.08 83.31μg/g. Conclusion The method was sensitive and accurate, and could be used for the quality control of wujin capsule.