手足口病患儿EV71的检测及分离鉴定

目的了解手足口病患儿感染EV71的情况,为科学防治疾病提供依据。方法采集手足口病患儿的咽拭子标本,接种于RD细胞,出现致细胞病变效应(CPE),运用RT-PCR方法,EV71引物检测样本和细胞培养物上清。结果标本直接用RT-PCR扩增,阳性率为30%(6/20),细胞培养后出现CPE的阳性率为45%(9/20),培养后经RT-PCR扩增阳性率为70%(14/20)。结论病毒分离和RT-PCR方法联合应用可以提高肠道病毒EV71的检出率,RT-PCR方法可以满足病毒流行期间同时处理大量样本的需要。

Detection and Identification of EV71 in Specimens from Children with hand-foot-mouth disease

Objective To understand the condition of infection of EV71 in a child with hand-foot-mouth disease,To provide the basis of science to prevent and cure diseases.Methods The throat swab specimen taken from a child with hand-foot-mouth disease was inoculated with RD cells,observing the cytopathic effect(CPE),RT-PCR was used to detect the EV71virus from specimen and cell culture supematant.Results The positive rate of throat swabs specimens was 30%(6/20)and increased to 70%(14/20)in the inoculated specimens.45%(9/20)of the inoculated specimens showed CPE.Conclusion The application of viral isolation plus RT-PCR can enhance detection rates of EV71,RT-PCR can meet the needs of the treatment of samples during an epidemic.