不同时间输注红细胞悬液对大鼠失血性休克复苏的影响

目的探讨红细胞悬液延迟输注30 min对大鼠失血性休克复苏的影响。方法 24只Wistar大鼠随机分为假手术(SHAM)、RBC 1和RBC 2组(n=8),RBC 1和RBC 2组制备重度失血性休克大鼠模型,先后采用晶胶复合液和红细胞悬液复苏,RBC 2组红细胞悬液延迟输注30 min;监测生理、血气等指标。结果与RBC1组相比,RBC 2组平均动脉压(MAP)和体温恢复延迟,RBC 1组复苏结束后2组MAP分别为(116.99±11.06)和(73.72±14.34)mm Hg(P0.01);RBC 1组复苏结束后30 min,2组MAP分别为(103.07±9.59)和(120.61±10.73)mmHg(P0.01)。RBC 1组复苏结束后120 min RBC 1组体温(37.28±0.80)℃明显低于RBC 2组(38.83±0.58)℃(P0.01)。与SHAM组相比,RBC 1和RBC 2组休克后pH、PCO2、BE和ctHb明显降低,PO2明显升高;复苏后PCO23 h,RBC 1和RBC 2组碱剩余(BE)值明显低于SHAM组。结论在失血性休克液体复苏的基础上,红细胞悬液延迟输注30 min对失血性休克大鼠模型的生理、血气等指标无明显影响。提示紧急救治中输血准备工作耗时可能不影响失血性休克的复苏效果。

Effects of different red blood cell transfusion time on hemorrhagic shock resuscitation in rat model

Objective This study aimed at investigating the effects of 30 minutes delay in red blood cell transfusion on hemorrhagic shock resuscitation in rat model.Methods 24 Wistar rats were randomized into 3 groups: sham hemorrhage group(SHAM);RBC 1group and RBC 2 group.RBC 1 and RBC 2 were prepared as severe hemorrhagic shock rat model and resuscitated with crystal-colloid fluid and red blood cells suspension.Red blood cells suspension transfusion of RBC 2 was delayed by 30 min.Physiological and blood gas parameters were monitored.Results Compared to RBC 1,the mean arterial pressure(MAP) and the body temperature of RBC 2 showed delays in recovery.At the time when resuscitation of RBC 1 was over,the MAPs of RBC 1 and RBC 2 were 116.99±11.06 mmHg and 73.72±14.34 mmHg(P<0.01),respectively.30 min after the resuscitation of RBC 1 was over,MAP of RBC 1 and RBC 2 were 103.07±9.59 mmHg and 120.61±10.73 mmHg(P<0.01) respectively.120 min after the resuscitation of RBC 1 was over,the body temperature of RBC 1(37.28±0.80)℃ was lower than that of RBC2(38.83±0.58)℃.Compared to the SHAM group,the pH,pCO2,BE and ctHb of both RBC 1 and RBC 2 declined after the hemorrhagic shock,while pO2 increased obviously.3h after resuscitation,base excess(BE) in RBC1 and RBC 2 were lower than that of SHAM.Conclusion 30 min delay in red blood cell transfusion following fluid resuscitation has no significant influence on the physiological and blood gas parameters in rat hemorrhagic shock model.The preparation time for blood transfusion during first aid may have no significance influence on the outcome of resuscitation from hemorrhagic shock.