| 氨氯地平衍生物CJX1对K562/DOX细胞阿霉素耐药的逆转作用 |
| |
| 引用本文： | 姬汴生,何玲,刘国卿.氨氯地平衍生物CJX1对K562/DOX细胞阿霉素耐药的逆转作用[J].中国药科大学学报,2005,36(2):159-163. |
| |
| 作者姓名： | 姬汴生 何玲 刘国卿 |
| |
| 作者单位： | 中国药科大学药理学教研室,南京,210009 |
| |
| 摘 要： | 目的:研究氨氯地平衍生物CJX1对K562/DOX细胞阿霉素耐药的逆转作用.方法:应用流式细胞仪和MTT法观察了CJX1对K562/DOX细胞P-糖蛋白(P-gp)的抑制作用及对K562/DOX细胞阿霉素耐药的逆转作用.结果:CJX1能剂量相关性地增加K562/DOX细胞内罗丹明123(Rh 123)的累计,明显抑制P-gp 介导的Rh 123外排,显著增强阿霉素对K562/DOX细胞的细胞毒作用,增加K562/DOX细胞内阿霉素水平.结论:氨氯地平衍生物CJX1能显著抑制P-gp的外排功能,逆转K562/DOX细胞的多药耐药性.
|
| 关 键 词： | 氨氯地平衍生物 P-糖蛋白 多药耐药 阿霉素 逆转作用 |
| 文章编号： | 1000-5048(2005)02-0159-05 |
| 修稿时间： | 2004年7月14日 |
Reversal of Resistance to Doxorubicin by CJX1,an Amlodipine Derivative,in Doxorubicin-resistant Human Myelogenous Leukemia (K562/ DOX) Cells |
| |
| JI Bian-Sheng,HE Ling,LIU Guo-Qing.Reversal of Resistance to Doxorubicin by CJX1,an Amlodipine Derivative,in Doxorubicin-resistant Human Myelogenous Leukemia (K562/ DOX) Cells[J].Journal of China Pharmaceutical University,2005,36(2):159-163. |
| |
| Authors: | JI Bian-Sheng HE Ling LIU Guo-Qing |
| |
| Abstract: | AIM:To study the reversal of resistance to doxoru bi cin by CJX1,an amlodipine derivative, in doxorubicin-resistant human myelogen o us leukemia(K562/DOX)cells.METHOD:The effect of CJX1 on P-glyco protein function and resistance to doxorubicin in K562/ DOX cells was explored by using flo w cytometric technology and MTT assay.RESULT:CJX1 increased intr acellular accumulation of rhodanmine 123 (Rh 123)in K562/DOX cells in a dose-de pendent manner and significantly inhibited the efflux of Rh 123 from the cells. The doxoru b icin-induced cytotoxicity and apoptosis were significantly potentiated by CJX1 by promoting intracellular doxorubicin.CONCLUSION:The reversed ef fect of CJX1 on multidrug resistance to doxorubicin in K562/ DOX cells could be atlributed to CJX1's inhibitory effect on P-glycoprotein function. |
| |
| Keywords: | CJX1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国药科大学学报》浏览原始摘要信息 |
| 点击此处可从《中国药科大学学报》下载免费的PDF全文 |
