选择性环氧合酶-2抑制剂对人类舌鳞癌Tea-8113细胞血管生成素蛋白表达的影响

目的研究选择性环氧合酶-2（COX-2）抑制剂尼美舒利（NIM）对人类舌鳞癌Tea-8113细胞血管生成素-1、2（Ang-1、Ang-2）蛋白表达的影响。方法体外培养人类舌鳞癌Tea-8113细胞，加入不同浓度的NIM（0、25、50、100、200μmol／L）进行干预，分别作用24、48、72h后，放射免疫法检测细胞培养上清液中PGE2分泌水平的改变，免疫组织化学（SP）方法检测人类舌鳞癌Tea-8113细胞内Ang-1和An舻2蛋白表达的变化情况。结果NIM呈浓度和时间依赖性地抑制PGE2分泌和Ang-2蛋白的表达，Ang-1蛋白表达受NIM影响不明显。结论NIM可减少PGE2分泌和Ang-2蛋白的表达，此机制可能是通过COX-2依赖性肿瘤血管生成抑制途径起作用。

Influence of COX-2 inhibitor on expression of Ang-1 and Ang-2 protein in human tongue squamous cell line Tca-8113

Objective To investigate the influence of Nimesulide(NIM),a selective Cyclooxygenase-2 (COX-2) inhibitor, on the expression of Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) protein in human tongue squamous cell carcinoma Tca-8113 cell lines. Methods Tca-8113 cell were cultured in vitro, and exposed to a range of concentrations of nimesulide (NIM) (0, 25, 50, 100, 200 μmol/L) for 24 h, 48 h, 72 h, and the PGE2 level in the cells cultured supernate was quantitated by the radioimmunoassay, and the expression of Ang-1 and Ang-2 protein in human tongue squamous cell line Tca-8113 were measured by the SABC immunocytochemistry staining simultaneously. Results The PGE2 level in the cultured supematant and expression of Ang-2 in Tca-8113 cells decreased by NIM in a concentration and time dependent manner, but not Ang-1. Conclusion NIM can decrease PGE2 level and Ang-2 expression, which may be involved in the inhibition of cancer angiogenesis of COX-2 dependence mechanisms.