| 噬菌体展示技术筛选日本血吸虫抱雌沟蛋白分子受体 |
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| 引用本文： | 何金桃,石艳丽,刘萍萍,刘金明,石耀军,林矫矫,金亚美. 噬菌体展示技术筛选日本血吸虫抱雌沟蛋白分子受体[J]. 中国血吸虫病防治杂志, 2011, 23(3): 279-284 |
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| 作者姓名： | 何金桃 石艳丽 刘萍萍 刘金明 石耀军 林矫矫 金亚美 |
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| 作者单位： | 1. 中国农业科学院上海兽医研究所、国家防治动物血吸虫病专业实验室、农业部动物寄生虫学重点开放实验室,上海,200241
2. 东北农业大学动物医学学院 |
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| 基金项目： | 国家自然科学基金(30800819); 上海市浦江人才计划(09PJ14118); 中央级公益性科研院所基本科研业务费(2010JB11) |
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| 摘 要： | 目的筛选日本血吸虫抱雌沟蛋白相互作用分子。方法利用噬菌体展示技术,以融合表达的抱雌沟蛋白为靶标,采用亲和淘洗方法筛选日本血吸虫44d成虫噬菌体展示cDNA文库。结果得到70条有效表达序列标签,对其进行聚类分析、同源性搜索与功能预测等生物信息学分析,获得5个与血吸虫抱雌沟蛋白有相互作用的蛋白或多肽。结论采用筛选噬菌体展示文库的方法成功获得抱雌沟蛋白相互作用分子。
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| 关 键 词： | 日本血吸虫 抱雌沟蛋白 筛选 噬菌体展示文库 |
Screening interaction protein of Schistosoma japonicum gynecophoral canal protein by phage display |
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| He Jin-tao,Shi Yan-li,Liu Ping-ping,Liu Jin-ming,Shi Yao-jun,Lin Jiao-jiao,Jin Ya-mei. Screening interaction protein of Schistosoma japonicum gynecophoral canal protein by phage display[J]. Chinese journal of schistosomiasis control, 2011, 23(3): 279-284 |
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| Authors: | He Jin-tao Shi Yan-li Liu Ping-ping Liu Jin-ming Shi Yao-jun Lin Jiao-jiao Jin Ya-mei |
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| Affiliation: | He Jin-tao1,Shi Yan-li2,Liu Ping-ping1,Liu Jin-ming1,Shi Yao-jun1,Lin Jiao-jiao1,Jin Ya-mei1* 1 Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Animal Parasitology,Ministry of Agriculture of China,Shanghai 200241,China,2 College of Veterinary Medicine,Northeast Agricultural University |
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| Abstract: | Objective To search the interaction protein of Schistosoma japonicum gynecophoral canal protein (SjGCP). Methods The recombinant rSjGCP was used as a target to search the T7 phage display cDNA library from 44-day adult Schistosoma japonicum. Results A total of 70 ESTs were obtained. The bioinformatics analyses to the screening results revealed that 5 interaction proteins or peptides of SjGCP were found. Conclusion Interaction proteins or peptides of SjGCP are successfully obtained through screening the T7 p... |
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| Keywords: | Schistosoma japonicum Gynecophoral canal protein Screening Phage display library |
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