链脲佐菌素联合烟酰胺诱导2型糖尿病大鼠模型的建立

目的：研究链脲佐菌素联合烟酰胺诱导2型糖尿病大鼠模型的方法。方法 Wistar大鼠隔夜禁食，腹腔注射烟酰胺60 mg/kg，15 min后腹腔注射链脲佐菌素65 mg/kg，在诱导后72 h、2周及4周观察大鼠行为、形态学改变，测量体重，空腹检测鼠尾静脉血糖，血糖大于11．1 mmol/L的大鼠即为诱导成功。结果诱导后的大鼠出现懒动、精神萎靡症状，与空白对照组相比体重下降（P＜0．05）、血糖上升（P＜0．05）；与单独使用链脲佐菌素诱导组相比体重上升（P＜0．05）、血糖较低（P＜0．05）。结论链脲佐菌素联合烟酰胺诱导糖尿病大鼠成功率高，是制备2型糖尿病大鼠模型的有效方法。

Streptozotocin-nicotinamide-induced type 2 diabetic model in rats

Objective To explore streptozotocin -nicotinamide-induced type 2 diabetic model in rats .Methods 3-month-old Wistar rats were fasted overnight then administered 60 mg/kg nicotinamide intraperitoneally （ i.p.） fol-lowed by an i.p.injection of 65 mg/kg streptozotocin 15 min later.After induced 72 hour, 2 weeks and 4 weeks, rats behavior and body weight were observed , and the fasting blood glucose level was determined in a sample taken from the tail vein of the rats.The diabetic status was confrmed by fasting hyperglycemia （fasting blood glucose≥11.1 mmol/L）. Results Ddiabetic model rats became lazy and distractive .Compared with the control group , the body weight after in-duction was lower （P〈0.05） with higher blood glucose level （P〈0.05）;but to the group induced only by streptozoto-cin, blood glucose after induced was decreased （P〈0.05） with significantly increased body weight （P〈0.05）.Con-clusion Streptozotocin-nicotinamide-induced diabetic rats provide an effective animal model for type 2 diabetes with high success rate .