| HPLC法测定注射用盐酸川芎嗪的含量 |
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| 引用本文： | 杨宜华,;王萌萌,;董凯,;张艳,;宋亚玲. HPLC法测定注射用盐酸川芎嗪的含量[J]. 徐州医学院学报, 2014, 0(5): 306-308 |
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| 作者姓名： | 杨宜华, 王萌萌, 董凯, 张艳, 宋亚玲 |
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| 作者单位： | [1]徐州医学院药学院药剂学教研室,江苏徐州221004; [2]徐州医学院药学院2010级;,江苏徐州221004; [3]徐州医学院药学院2011级,江苏徐州221004; |
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| 基金项目： | 基金项目：徐州市科技计划项目（XZZD1225）,江苏省大学生实践创新训练计划项目（2012JSSPITP1869） |
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| 摘 要： | 目的采用高效液相色谱法（HPLC）测定注射用盐酸川芎嗪中盐酸川芎嗪的含量。方法采用HY—PERSILODSC18柱（250mm×4．6mm，5μm），流动相为甲醇：三乙胺水溶液（0．2％，冰醋酸调pH至5．00）（60：40），流速1．0ml·min^-1，柱温为室温，检测波长295nm。结果盐酸川芎嗪在4～44μg·ml。线性关系良好（r=0．9999）。盐酸川芎嗪平均回收率为101．25％±4．03％；平均含量为92．43％±0．36％。结论该法专属性好、操作简便、快速准确，可测定注射用盐酸川芎嗪中盐酸川芎嗪的含量。
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| 关 键 词： | 盐酸川芎嗪 高效液相色谱法 含量测定 |
Determination of ligustrazine hydrocholride injection by HPLC |
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| Affiliation: | YANG Yihua, WANG Mengmeng, DONG Kai, ZHANG Yan, SONG Yaling (1. School of Pharmacy, Xuzhou Medical College, Xuzhou, Jiangsu 221004, China; 2. Grade 2010, School of Pharmacy, Xuzhou Medical College; 3. Grade 2011, School of Pharmacy, Xuzhou Medical College) |
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| Abstract: | Objective To establish a HPLC method for determination of ligustrazine hydrochloride injection. Methods A HYPERSIL ODS C18 （250 mm × 4.6 mm, 5 μm） column was employed in the current study. The mobile phase consisted of methanol and triethylamin （0.2%o , adjusted with glacial acetic acid to pH 5.00） （40：60） , with a flow rate of 1.0 ml/min and a detection wavelength of 295 nm at room temperature. Results A good linear relationship was acquired when the concentration of ligustrazine hydrochloride was within the range of 4 - 44 mg/L （ r = 0. 9999）. The average recovery was 101.25% ± 4.03%. The average content was 92.43% ± 0.36%. Conclusion The method was convenient, fast and accurate for determining the content of ligustrazine hydrochloride injection, with high specificity. |
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| Keywords: | ligustrazine hydrochloride HPLC content determination |
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