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| 草乌中生物碱含量测定方法的研究 | |
| 引用本文： | 黄建明,郭济贤,孙明明,陆斌,段更利,陈万生. 草乌中生物碱含量测定方法的研究[J]. 中药材, 2002, 25(12): 878-880 |
| 作者姓名： | 黄建明 郭济贤 孙明明 陆斌 段更利 陈万生 |
| 作者单位： | 1. 复旦大学药学院,上海200032 2. 第二军医大学药学院,上海200433 |
| 基金项目： | 复旦大学研究生创新基金资助项目 |
| 摘 要： | 研究草乌中乌头类生物碱的含量测定方法,并对草乌生药进行测定。总生物碱的含量采用容量法和酸性染料比色法测定;容量法采用硫酸回滴法,以甲基红-溴甲酚绿为指示剂;酸性染料比色法采用溴甲酚绿法,检测波长416 nm。酯型总生物碱的含量采用异羟肟酸铁比色法测定。三种双酯型生物碱的含量采用高效液相色谱法测定,固定相为C18,流动相为乙腈:缓冲液(冰醋酸-三乙胺,pH6.25)=71:29,检测波长235 nm。 |
| 关 键 词： | 草乌 乌头类生物碱 含量测定 |
| 修稿时间： | 2002-08-20 |
The Method for Determination of Aconitum Alkaloids in Radix Aconiti Kusnezoffii |
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| Huang Jianming,Guo Jixian,Sun Mingming,Lu Bin,Duan Gengli. The Method for Determination of Aconitum Alkaloids in Radix Aconiti Kusnezoffii[J]. Journal of Chinese medicinal materials, 2002, 25(12): 878-880 | |
| Authors: | Huang Jianming Guo Jixian Sun Mingming Lu Bin Duan Gengli |
| Affiliation: | School of Pharmacy, Fudan University, Shanghai 200032. |
| Abstract: | The methods for the determination of Aconitum alkaloids in Radix Aconiti Kusnezoffii were established. Volumetric analysis with the indicator of methyl red-bromcresol green and colonmetric analysis with acid dye of bromecresol green under the wavelength of 416nm were used for the assay of total alkaloids. Colonmetric analysis of hydroxamic acid-Fe was for the assay of total ester-diterpene type Aconitum alkaloids. Three kinds of diester-diterpene type Aconitum alkaloids (aconitine, hypaconitine and mesaconitine) were determined by HPLC method.The HPLC system consisted of C18 as the stationary phase and acetonitrile;buffer (acetic acid-triethylamine,pH6.25) = 71:29 as the mobile phase.The wavelength was 235 nm. |
| Keywords: | Aconitum kusnezoffu Aconitum alkaloids Determination |
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