补肾活血化痰法对拟血管性痴呆小鼠脑组织病理形态学及谷氨酸含量的影响

目的观察补肾活血化痰法对拟血管性痴呆小鼠脑组织病理形态学及谷氨酸(Glu)含量的影响,进而探讨其作用机制。方法制作脑部缺血再灌注拟血管性痴呆小鼠模型。术后第15天对各组小鼠脑组织进行病理形态学观察并检测Glu含量。结果补肾活血化痰组海马CA1区细胞线较清晰,细胞排列紧密,数量明显增多;且该组小鼠脑组织内Glu含量明显降低。结论补肾活血化痰法能抑制小鼠缺血脑组织中Glu的释放,降低其兴奋性,从而保护神经元。这可能是补肾活血化痰法治疗血管性痴呆的作用机制之一。

Effects of invigorating kidney, promoting blood and dissipating phlegm on the pathomorphology and Glu content of brain tissues of mice with analog vascular dementia

Objective To observe the effects of invigorating kidney, promoting blood and dissipating phlegm on the pathomorphology and Glu content of brain tissues of mice with analog vascular dementia, and to further explore the action mechanism of this method. Methods The model mice with analog vascular dementia were made by ischemia-reperfusion in the brain. 15 days after the operation, the pathomorphology was observed and Glu content in the brain tissue of mice in all groups was detected. Results In the invigorating kidney, promoting blood and dissipating phlegm group, the cell lines in CA1 area of hippocampus were relatively clear, the arrangement of cells was relatively compact and the amount of cells increased obviously. Glu content in the brain tissue obviously lowered. Conclusions Method of invigorating kidney, promoting blood and dissipating phlegm is able to protect neurons through inhibiting the release of Glu in the brain tissues of mice, thus lowering its excitotoxicity. It may be part action mechanism of this method to treat vascular dementia.