| 高效液相色谱法测定青藤碱微乳中青藤碱含量 |
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| 引用本文： | 唐志刚,朱红燕. 高效液相色谱法测定青藤碱微乳中青藤碱含量[J]. 中国交通医学杂志, 2011, 0(5): 453-455 |
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| 作者姓名： | 唐志刚 朱红燕 |
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| 作者单位： | 南通大学附属医院,江苏226001 |
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| 摘 要： | 目的:探讨建立高效液相色谱法测定青藤碱微乳中青藤碱含量的方法。方法:采用ODS-C18色谱柱,以乙腈-0.01%磷酸二氢钾溶液(用三乙胺调pH至7.8)(40∶60)为流动相,检测波长为262 nm,流速1.0 mL/min,柱温为室温,进样量为20μL。结果:青藤碱在40.6~203μg/mL范围内线性关系良好,线性回归方程:A=9 934.5C+9 839.8(r=0.9999),平均回收率为102.36%(RSD=4.02%)。结论:高效液相色谱法简便、快速、灵敏、重复性好,可作为青藤碱微乳含量测定方法。
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| 关 键 词： | 青藤碱 高效液相色谱法测定 微乳 |
Determination of microemulsion in sinomenine by HPLC |
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| TANG Zhigang,ZHU Hongyan. Determination of microemulsion in sinomenine by HPLC[J]. Chinese Medical JOurnal of Communications, 2011, 0(5): 453-455 |
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| Authors: | TANG Zhigang ZHU Hongyan |
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| Affiliation: | (Affiliated Hospital of Nantong University,Jiangsu 226001) |
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| Abstract: | Objective： To establish a HPLC method for the determination of sinomenine microemulsion.Methods： An intersil ODS-C18 column was used with the mobile phase acetonitrile-0.1% potassium dihydrogen phosphate（pH7.8 adjusted by triethylamine）（40：60） at a detection wavelength 262 in an injection volume 20μl;The flow rate was 1.0ml/min and column temperature was room temperature;Results： sinomenine had good linear relation in the range of 40.6-203μg/ml.The average recovery was 102.36%（RSD=4.02%）.Conclusion： HPLC is simple,fast,sensitive and fit for the concentration control of the dispersible tablets. |
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| Keywords: | Sinomenine High performance liquid chromatography Microemulsion Content |
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