锰对新生鼠肝组织HSP70合成的影响

雌性大鼠随机分为4组,腹腔注射氯化锰两周,隔日一次,剂量分为0、75、15、30mg/kg.受孕后以同样方法继续染锰一周.分娩后对其新生鼠肝组织用western斑点印迹法检测热应激蛋白70(HSP70),并测定其超氧化物歧化酶(SOD).发现15mg/kg组SOD活性低于对照组(P<0.05);HSP70含量,染锰各组明显高于对照组(P<0.01).提示亲代妊娠期染锰可诱导子代新生鼠肝组织HSP70合成增加,但这种效应在锰的胚胎发育毒性中的作用和机理仍有待进一步阐明.

Effect of Manganese(Mn) on Heat Stress Protein 70 Synthesis in the Liver of New-born Rats

female rats were divided into four groups.One group took as control,the other three groups received.manganese chloride(MnCl-2) as intraperitioneal injection every two days for two weeks,the dose were 7 5,15 and 30 mg/kg respectively.After pregnant the mother rats received MnCl-2 unceasingly for a week with the same method as before.The levels of HSP70 in the livers of the new born rats were determined with western dot blotting,and the activity of SOD were determined simultaneously.The activity of SOD in the livers of new born rats of 15mg/kg group were lower than the control group (P<0 05);the levels of HSP70 in the livers of new born rats of three groups that mother rats exposed to the manganeses increased significantly(P<0 01) compared with the control.Exposure to MnCl-2 of mother rats could induce HSP70 synthesis in the livers of new born rats,but the mechanism of this effect in the embryonic developtoxicity of Mn remain to be delineated.