| 利用噬菌体肽库筛选脂多糖的模拟表位 |
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| 引用本文： | 董洁,高亚萍,张杰,柳川,薛沿宁. 利用噬菌体肽库筛选脂多糖的模拟表位[J]. 军事医学科学院院刊, 2003, 27(4): 271-273 |
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| 作者姓名： | 董洁 高亚萍 张杰 柳川 薛沿宁 |
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| 作者单位： | 军事医学科学院基础医学研究所,北京,100850 |
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| 摘 要： | 目的：利用噬菌体肽库获得脂多糖的模拟表位。方法：用脂多糖免疫大耳白兔获得抗血清，从中纯化抗脂多糖的多克隆抗体。以纯化的抗体IgG为筛选靶分子，对噬菌体随机十二肽库进行亲和筛选。经3轮筛选后，随机挑取25个克隆测序，并进行噬菌体ELISA结合实验及脂多糖竞争抑制实验，以确定阳性克隆。结果：获得12种序列，ELISA实验结果显示其中6个为阳性克隆，且经脂多糖竞争抑制实验进一步证实。结论：获得了6个脂多糖的模拟表位，为脂多糖抗原表位的研究及疫苗研究提供了线索。
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| 关 键 词： | 脂多糖 噬菌体随机肽库 模拟表位 筛选 |
| 文章编号： | 1000-5501(2003)04-0271-03 |
| 修稿时间： | 2002-10-08 |
Screening of LPS mimotope by phage-displayed peptide library |
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| DONG Jie,GAO Ya_Ping,ZHANG Jie,LIU Chuan,XUE Yan_Ning. Screening of LPS mimotope by phage-displayed peptide library[J]. Bulletin of the Academy of Military Medical Sciences, 2003, 27(4): 271-273 |
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| Authors: | DONG Jie GAO Ya_Ping ZHANG Jie LIU Chuan XUE Yan_Ning |
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| Abstract: | Objective:To obtain mimotopes of LPS by phage_displayed random peptide library.Methods:Polyclonal IgG antibody was obtained by immunizing rabbits and anti_sera were purified. The specific polyclonal antibody was used as the target for biopanning of a 12_mer phage_displayed random peptide library. After 3 rounds of screening, 25 clones were randomly selected for DNA sequencing, then detected by ELISA and LPS competition experiments to identify the positive clones.Results:Twelve sequences were obtained. Six of twelve clones were proved to be positive, and confirmed by competition experiments.Conclusion:The six phage_displayed peptides as mimotope of LPS were obtained. The findings provide a clue to LPS epitope mapping and vaccine development. [ |
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| Keywords: | lipopolysaccharide phage_displayed random peptide library mimotope |
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