环氧合酶-2抑制剂对人结肠癌细胞增殖及侵袭力影响的研究

目的 研究环氧合酶-2(COX-2)抑制剂SC236对人结肠癌细胞株增殖及侵袭能力的影响,探讨COX-2抑制剂用于结肠癌转移治疗的可能性.方法 人结肠癌细胞株LS-174T经环氧合酶-2抑制剂SC236处理后,用MTT法检测其增殖能力的变化,用黏附基质分析法和Transwell法检测其侵袭能力的变化,并通过免疫组化方法检测细胞中COX-2、VEGF及基质金属蛋白酶-2(MMP-2)蛋白的表达.结果 经SC236处理后,LS-174T细胞的增殖受到抑制,侵袭能力和黏附率下降,运动能力减低,细胞中COX-2、VEGF及MMP-2蛋白的表达减低.结论 SC236能通过COX-2途径抑制结肠癌细胞的生长和侵袭,其机制可能与其抑制肿瘤的血管生成、减低MMP-2的表达有关.

Study on effect of cox-2 inhibitor on proliferation and invasion of human colon carcinoma cells

Objective To explore the effect of cox-2 inhibitor on the proliferation and invasion of human colon carcinoma.Methods After treatment of LS-174T cells with SC236,MTT assay was used to test the effect on the proliferation.Cell biologic behaviors such as adhesiveness,cell motility and in vitro invasiveness were observed.Immunohistochemical methord was used to detect the COX-2,VEGF and MMP-2 expression.Results The suppressive effect of SC236 on the proliferation of LS-174T was dependent on time and concentrati...