|
|||||
|
|
| PDLLA-T3对体外培养雪旺细胞影响的实验研究 | |
| 引用本文： | 王敏,杨志焕,潘君,Maquet V. PDLLA-T3对体外培养雪旺细胞影响的实验研究[J]. 重庆医学, 2005, 34(8): 1184-1186,1189 |
| 作者姓名： | 王敏 杨志焕 潘君 Maquet V |
| 作者单位： | 第三军医大学新桥医院骨科,重庆,400037;第三军医大学大坪医院野战外科研究所,重庆,400042;重庆大学生物力学与组织工程重点实验室,400030;比利时列日大学化学系大分子研究教育中心 |
| 摘 要： | 目的观察甲状腺激素人工神经(PDLLA-T3)对体外培养雪旺细胞的影响.方法体外纯化、培养雪旺细胞后,在雪旺细胞培养板中加入PDLLA-T3(C组),混合培养6d,实验采用MTT法每天观察细胞活性的高低,用血球计数板对雪旺细胞进行计数,并用ELISA方法测定每天培养液中NGF含量的变化.另有不合T3的支架材料PDLLA(B组)、空白组(A组)作为实验对照组.结果培养的雪旺细胞纯度达到94.63%.PDLLA-T3比PDLLA和空白组明显增加雪旺细胞的数量,提高细胞活性,刺激雪旺细胞产生更多的NGF.结论 PDLLA-T3和雪旺细胞有较好的组织相容性,对培养的雪旺细胞有较好的正性促进作用,是一种良好的组织工程支架. |
| 关 键 词： | 雪旺细胞 聚乳酸 组织工程 NGF 三碘甲状腺原氨酸 |
| 文章编号： | 1671-8348（2005）08-1184-04 |
The effects of PDLLA-T3 on cultured schwann cells |
|
| WANG Min,YANG Zhi-huan,PAN Jun,Maquet V. The effects of PDLLA-T3 on cultured schwann cells[J]. Chongqing Medical Journal, 2005, 34(8): 1184-1186,1189 | |
| Authors: | WANG Min YANG Zhi-huan PAN Jun Maquet V |
| Abstract: | Objective To observe the effects of PDLLA-T3 on cultured Schwann cells. Methods Schwann cells were cultured with PDLLA-T3 for 6d in vitro, and every day the activities of cells, the number of the cells and contents of NGF were detected by the methods of MTT experience, erytbrornetry and immunobistocbemistry respectively, the scaffold with no triidotbyronine and cultures with no any scaffold were carried out as contrast. Results The purity of Scbwann cells in cultures was 94. 63%. more number of cells, more activity of cells and more contents of NGF were observed in PDLLA-T3 than those in other groups. Conclusion Compatibility of the PDLLA-T3 in Schwann cells were perfect. PDLLA-T3 has positive effect on cultured Schwann cells and is a fine scaffold for peirpheral nerve. |
| Keywords: | schwann cells polylactide acide tissue engineering neutrophic growth factor triidiothyronine |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |