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The role of acentric chromosome fragments in gene amplification |
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| Authors: | Peter Hahn William F. Morgan Robert B. Painter |
| Affiliation: | (1) Laboratory of Radiobiology and Environmental Health, University of California, 94143 San Francisco, California;(2) Department of Microbiology, University of California, 94143 San Francisco, California;(3) Present address: Department of Radiology, Division of Radiation Oncology, State University Hospital of the Upstate Medical Center, 750 Adams Street, 13210 Syracuse, New York |
| Abstract: | We assessed the role of acentric chromosome fragments in gene amplification by using cell fusion techniques to introduce the fragmented chromosomes of a donor Chinese hamster ovary (CHO) cell line that contained the dihydrofolate reductase (dhfr)gene(s) into a CHO cell line deficient for dhfr.Chromosome fragments were successfully integrated into cells at a frequency of approximately 3%. Methotrexate-resistant variants arose much more frequently in two cell lines derived from these successful cell fusions than in wild-type CHO cells. The hybrid cell lines also amplified their dhfrgenes more readily than did the CHO cell line used as dhfrdonor. |
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