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| 4种支架构建复合式口腔黏膜的组织学特点 | |
| 引用本文： | 刘加荣,JW Von den Hoff,陈莉莉. 4种支架构建复合式口腔黏膜的组织学特点[J]. 华中科技大学学报(医学版), 2011, 40(5): 558-561. DOI: 10.3870/j.issn.1672-0741.2011.05.014 |
| 作者姓名： | 刘加荣 JW Von den Hoff 陈莉莉 |
| 作者单位： | 1. 华中科技大学同济医学院附属协和医院口腔科,武汉,430022;荷兰Radboud大学医学中心,正畸与颅面生物系,PO Box 9101,6500,HB Nijmegen 2. 荷兰Radboud大学医学中心,正畸与颅面生物系,PO Box 9101,6500,HB Nijmegen 3. 华中科技大学同济医学院附属协和医院口腔科,武汉,430022 |
| 基金项目： | 国家自然科学基金，湖北省卫生厅科研基金 |
| 摘 要： | 目的利用4种不同支架材料构建复合式口腔黏膜,并比较其组织结构特点。方法体外培养人口腔黏膜的成纤维细胞和角质形成细胞,在4种支架材料中加入成纤维细胞,培养7d后,在支架表面加入角质形成细胞,培养4d后,移至气-液界面继续培养7d。苏木精-伊红染色镜下观察构建的复合式口腔黏膜的组织形态学特点。结果 4种支架均可构建形成复层上皮。其中,上皮层与脱细胞真皮基质材料(de-epidermised dermis,DED)结合紧密,形成的人工黏膜有明显的上皮钉突。不同于以往报道,上皮层与Alloderm结合并不十分紧密。以胶原凝胶为基质形成的人工口腔黏膜最厚,有明显分层。以胶原海绵-胶原凝胶为基质形成的复层上皮在部分区域长入至胶原海绵的空隙中。结论以DED和胶原凝胶为支架构建的口腔黏膜更接近于天然结构,而后者脆性较大,限制了其临床应用的可能。 |
| 关 键 词： | 组织工程口腔黏膜 支架材料 成纤维细胞 角质形成细胞 |
Characteristics of Tissue Engineered Oral Mucosa Based on Four Different Scaffolds |
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| Liu Jiarong,,JW Von den Hoff,AM Kuijpers-Jagtman et al. Characteristics of Tissue Engineered Oral Mucosa Based on Four Different Scaffolds[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2011, 40(5): 558-561. DOI: 10.3870/j.issn.1672-0741.2011.05.014 | |
| Authors: | Liu Jiarong JW Von den Hoff AM Kuijpers-Jagtman et al |
| Affiliation: | Liu Jiarong1,2,JW Von den Hoff2,AM Kuijpers-Jagtman2 et al 1Department of Stomatology,Union Hospital,Tongji Medical College,Huazhong University of Science and Tethnology,Wuhan 430022,China 2Department of Orthodontics and Craniofacial Biology,Radboud University Nijmegen Medical Centre,Nijmegen,The Netherlands |
| Abstract: | Objective To develop,optimize and characterize tissue engineered oral mucosa equivalents made of an epithelium layer on 4 different scaffolds. Methods Fibroblasts and keratinocytes were isolated and cultured from mucosa biopsies obtained from healthy volunteers.After amplification,fibroblasts were seeded into de-epidermized human dermis(DED)and Alloderm by centrifugation.The collagen based scaffolds were prepared by seeding fibroblasts directly.After one week culture,the keratinocytes were seeded on top of ... |
| Keywords: | tissue engineered oral mucosa scaffold fibroblasts keratinocytes |
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