输精管结扎鼠睾丸ABP和核雄激素受体的动态变化研究

雄性Wistar大鼠 60只,分为输精管结扎后 3月组(V—3),8月组(V—8),15月组(V—15)及其相应对照组(C—3,C—8,C—15)各10只。血清睾酮(ST),睾丸组织睾酮(TT),睾丸雌激素结合蛋白(ABP)和细胞核雄激素受体(NAR)水平检测结果是:①V—15组TT显著高于C—15组(P<0.01),其余组间比较及ST各组间比较均无显著差异(P>0.05);②ABP的平衡解离常数(Kd值),V—3组高于C—3组(P<0.05),但各组都在1.38～5.26nmol范围内,ABP水平结扎组高于相应对照组(P<0.05);③NAR的平衡解离常数各组间无明显差异(P>0.05),NAR水平对应组间无显著差异,但3个结扎组间比较V—8组最低(P<0.05)。

Dynamic Changes of ABP and nuclear androgen receptor in rat testis after vasectomy

Levels of serum testosterone (ST). intratesticular testosterone (TT). androgen binding protein (ABP) and nuclear androgen receptor (NAR) of testicular tissue in Wistar rats were studied in 3 (V-3). 8 (V-8) and 15 (V-15) months following vasectomy and the corresponding control groups C-3, C-8 and C-15. The restults showed that: (1) there were no significant differences in ST and TT among comparable groups, except TT between V-15 and C-15: (2) the Kd value of ABP in V-3 was significantly larger than that in C-3. with no significant differences among the rest comparable groups. The Bmax values by Scatchard analysis and the levels of ABP by single point analysis in V - groups were obviously higher than those in C -groups: (3) the Kd values and concentrations of NAR in V -groups were not significantly different from those in C -groups.