| Effects of Fat-soluble Extracts From Vegetable Powder and β-carotene on Proliferation and Apoptosis of Lung Cancer Cell YTMLC-90 |
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| 作者姓名： | Lu QJ Huang CY Yao SX Wang RS Wu XN |
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| 作者单位： | [1]DepartmentofNutritionandFoodHygiene,CollegeofPublicHealth,ZhengzhouUniversity,Zhengzhou450052,Henan,China [2]DepartmentofNutritionandFoodHygiene,WestChinaSchoolofPublicHealth,SichuanUniversity,Chengdu610041,Sichuan,China [3]YunnanTinMinerCorporation,GejiuCity661400,Yunnan,China [4]DepartmentofClinicalNutrition,People‘sHospitalofSichuanProvince,Chengdu610031,Sichuan,China |
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| 摘 要： | Objective The aim of this investigation was to study the effects of fat-soluble extracts from vegetable powder (FEFVP) and β-carotene on the proliferation and apoptosis of cultured YTMLC-90 lung cancer cells. Methods The lung cancer cells were continuously exposed to a broad range of concentration of FEFVP and 3-carotene. The proliferation was evaluated in MTT test. The induction of apoptosis was evaluated by morphological change, DNA fragmentation analysis, and DNA content analysis combined with flow cytometric analysis. Results Both FEFVP and β-carotene were found to inhibit cell proliferation and to induce morphologic changes consistent with apoptosis in YTMLC-90 cancer cells, including cellular shrinkage, chromatin condensation and nuclear fragmentation. DNA agarose gel electrophoresis showed DNA fragmentation ‘ladder‘‘. Flow cytometric analysis revealed decreased DNA content and the presence of a sub-G1 apoptotic peak.Conclusion These findings are consistent with the induction of apoptosis. Moreover, the effects of FEFVP are stronger than those of β-carotene. FEFVP inhibits the growth of YTMLC-90 probably via the induction of apoptosis cancer cells.
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| 关 键 词： | β-胡罗卜素 脂溶性提取物 蔬菜 细胞增殖 细胞凋亡 肺癌 作用机制 |
Effects of fat-soluble extracts from vegetable powder and beta-carotene on proliferation and apoptosis of lung cancer cell YTMLC-90 |
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| Lu QJ,Huang CY,Yao SX,Wang RS,Wu XN.Effects of fat-soluble extracts from vegetable powder and beta-carotene on proliferation and apoptosis of lung cancer cell YTMLC-90[J].Biomedical and Environmental Sciences,2003,16(3):237-245. |
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| Authors: | Lu Quan-Jun Huang Cheng-Yu Yao Shu-Xiang Wang Rui-Shu Wu Xiao-Na |
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| Institution: | Department of Nutrition and Food Hygiene, College of Public Health, Zhengzhou University, Zhengzhou 450052, Henan, China. lqjnutr@zzu.edu.cn |
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| Abstract: | Objective The aim of this investigation was to study the effects of fat-soluble extracts from vegetable powder (FEFVP) and P-carotene on the proliferation and apoptosis of cultured YTMLC-90 lung cancer cells. Methods The lung cancer cells were continuously exposed to a broad range of concentration of FEFVP and P-carotene. The proliferation was evaluated in MTT test. The induction of apoptosis was evaluated by morphological change, DNA fragmentation analysis, and DNA content analysis combined with flow cytometric analysis. Results Both FEFVP and P-carotene were found to inhibit cell proliferation and to induce morphologic changes consistent with apoptosis in YTMLC-90 cancer cells, including cellular shrinkage, chromatin condensation and nuclear fragmentation. DNA agarose gel electrophoresis showed DNA fragmentation 'ladder'. Flow cytometric analysis revealed decreased DNA content and the presence of a sub-G1 apoptotic peak. Conclusion These findings are consistent with the induction of apoptosis. Moreover, the effects of FEFVP are stronger than those of P-carotene. FEFVP inhibits the growth of YTMLC-90 probably via the induction of apoptosis cancer cells. |
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| Keywords: | Cell culture FEFVP Apoptosis |
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