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| IL-18、IL-12及相关因子在大鼠实验性自身免疫心肌炎心肌中的表达时程和特征 | |
| 引用本文： | 常贺,李刚,张乐,邹军,朱铉,金鑫. IL-18、IL-12及相关因子在大鼠实验性自身免疫心肌炎心肌中的表达时程和特征[J]. 免疫学杂志, 2010, 0(6) |
| 作者姓名： | 常贺 李刚 张乐 邹军 朱铉 金鑫 |
| 作者单位： | 厦门大学附属中山医院心内科;厦门大学医学院药学系; |
| 基金项目： | 国家自然基金资助项目(30670512);;教育部留学归国启动经费(教外司留[2008]890号) |
| 摘 要： | 目的:探讨IL-18、IL-12及相关细胞因子在大鼠实验性自身免疫心肌炎(EAM)心肌中的表达时程和特征。方法:以猪心脏肌球蛋白加等体积弗氏完全佐剂,辅结核杆菌H37Ra株之乳液为抗原,于Lewis大鼠双后肢皮下注射造模(EAM);用胶原酶灌流和网筛过滤法分离纯化心肌细胞;经免疫组化技术评估心肌损伤程度;按实时荧光定量法测急性期和慢性期,IL-18、IL-12及相关因子(IL-18R,IL-18RAcPL,IL-18BP以及IL-12p40,IL-12p35,IL-12Rβ1,IL-12Rβ2)mRNA表达情况。结果:IL-18、IL-12及相关因子表达主要在急性期,免疫后2周表达增多并达峰值(P<0.01,与正常对照组比较),4周后减少,并与EAM病程正相关;IL-18、IL-12及相关因子主要在巨噬细胞表达,其受体复合物在αβT细胞表达;慢性期仅IL-18BP在心肌细胞有表达。结论:IL-18、IL-12及相关因子参与EAM病理过程,各因子主要集中于急性期巨噬细胞和αβT细胞表达。 |
| 关 键 词： | IL-18 IL-12 实验性自身免疫性心肌炎 实时荧光定量RT-PCR |
Time course and properties of myocardial expression of IL-18, IL-12, and related cytokines in rat experimental autoimmune myocarditis |
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| CHANG He,LI Gang,ZHANG Le,ZOU Jun,ZHU Xuan,JIN Xin. Time course and properties of myocardial expression of IL-18, IL-12, and related cytokines in rat experimental autoimmune myocarditis[J]. Immunological Journal, 2010, 0(6) | |
| Authors: | CHANG He LI Gang ZHANG Le ZOU Jun ZHU Xuan JIN Xin |
| Affiliation: | CHANG He,LI Gang,ZHANG Le,ZOU Jun,ZHU Xuan,JIN Xin Department of Cardiology,Zhongshan Hospital Affiliated to Xiamen University,Xiamen 361005,China |
| Abstract: | To explore the time course and properties of myocardial expressions of IL-18, IL-12, and related cytokines in rat experimental autoimmune myocarditis (EAM), an antigen was prepared with an emulsion of porcine cardiac myosin in isopyknic complete Freund's adjuvant, plus Mycobecterium tuberculosis H37Ra strain. EAM model was made by hypodermic injection of the antigen in hind legs of Lewis rats. Then the isolation and purification of cardiocytes were carried out through the methods of collagenase perfusion an... |
| Keywords: | IL-18 IL-12 Experimental autoimmune myocarditis Real-time fluorescence quantitative RT-PCR |
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