| 泰安市甲型H3N2流感病毒HA1氨基酸变异特点分析 |
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| 引用本文： | 刘运强,侯佩强,陆娟娟,张荣强.泰安市甲型H3N2流感病毒HA1氨基酸变异特点分析[J].中国病原生物学杂志,2011(3). |
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| 作者姓名： | 刘运强 侯佩强 陆娟娟 张荣强 |
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| 作者单位： | 山东省泰安市疾病预防控制中心; |
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| 基金项目： | 泰安市科学技术发展计划一般项目(No.20071034) |
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| 摘 要： | 目的了解2006~2008年泰安市甲型H3N2流感病毒HA1基因变异特征。方法采集本地区流感样病例咽拭子,分离病毒,选择甲型H3N2流感病毒,提取核酸,采用逆转录-聚合酶链反应(RT-PCR)扩增并测序,推导其编码氨基酸序列,进行基因进化特征分析。结果 2006~2008年共检测咽拭子524份,分离出流感病毒119株,分离阳性率为22.71%。119株流感病毒中H3N2亚型65株,H1N1亚型2株,B型Victoria系13株,B型Yamagata系39株。对8株H3N2病毒进行基因进化树分析,在其推导HA1蛋白分子抗原决定簇A上有3个氨基酸位点(R142G、N144D和I140K)发生突变。结论在近2个年度的流行季节中,本地区以H3N2亚型和B型Yamagata系为优势毒株,也有B型Victoria系和甲型H1N1亚型的存在。泰山分离株HA1区发生氨基酸替换的位点较少。WHO推荐A/Wisconsin/67/2005为北半球2006~2008年度的流感疫苗株,泰山分离株与此疫苗株的相似性较高,因此认为该流感疫苗对当年度优势株H3N2流感病毒的预防有一定效果。
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| 关 键 词： | 流感病毒 细胞培养 聚合酶链反应 基因序列 抗原位点 |
Research on the genetic characteristics of HA1 in strains of the influenza A(H3N2) virus isolated in Taian City |
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| LIU Yun-qiang,HOU Pei-qiang,LU Juan-juan,ZHANG Rong-qiang.Research on the genetic characteristics of HA1 in strains of the influenza A(H3N2) virus isolated in Taian City[J].Journal of Pathogen Biology,2011(3). |
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| Authors: | LIU Yun-qiang HOU Pei-qiang LU Juan-juan ZHANG Rong-qiang |
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| Abstract: | Objective To ascertain the characteristics of genetic variation in HA1 of the influenza A(H3N2) virus isolated in Tai'an City from 2006-2008.Methods Nasopharyngeal swabs were collected from patients with influenza-like illness(ILI).The virus was isolated with MDCK cells and identified with HAI and RT-PCR.PCR products were sequenced.The sequences were analyzed using biometric software.Results One hundred and nineteen strains of influenza were identified in 524 specimens from Tai'an City from 2006-2008,and sp... |
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| Keywords: | Influenza virus cell culture PCR nucleotide sequence antigen site |
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